Abstract <p>Biodiesel market in Mexico still faces some challenges due to its high production costs. An additional challenge that may arise is the consequent excessive production of glycerol. Therefore, the glycerol valorization into high value-added chemical products is necessary to enhance the economic potential of the biodiesel industry. In this work, a mathematical model representing the supply chain for the valorization of crude glycerol from the biodiesel industry in Mexico is developed. The proposed model involves operational and logistical decisions such as the selection of high value-added chemical products, biorefinery locations, and production volumes. Furthermore, the model analyzes the impact of variations in selling prices, product demand, and the availability of glycerol on the optimal supply chain configuration. According to the results, the maximum profit implies only the production of lactic acid (LA) in Mexico City and the state of Nuevo Leon, satisfying less than 4% of the total demand. Additionally, it is observed that a greater availability of glycerol would increase the amount of demand satisfied and its use could strengthen the biodiesel industry in Mexico.</p> Graphic abstract <p></p>