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Research background <p> Metagenomic next-generation sequencing (mNGS) is a culture-independent pathogen identification method, which can directly sequence all nucleic acids present in clinical samples, and has shown transformative potential in the diagnostic field of complex, critical and emerging infectious diseases, but its clinical application value has not been fully evaluated. This study aims to compare the diagnostic efficacy of mNGS and traditional microbiological testing (TMT), and evaluate its impact on clinical decision-making.</p> Research methods <p> This retrospective study analyzed the data of the laboratory information system (LIS) of patients who received both mNGS and TMT testing.</p> Research results <p> In samples of bronchoalveolar lavage fluid (BALF), blood and cerebrospinal fluid (CSF), the positive rates of mNGS were 86.70%, 77.17% and 53.57% respectively, which were significantly higher than the corresponding positive rates of TMT (41.38%, 14.13%, 17.86%). Clinical correlation analysis showed that 77.84%, 66.20% and 73.33% of the positive mNGS results of the three types of samples were of clinical significance respectively. 15.34%~29.58% of the cases adjusted their treatment regimens according to the positive mNGS results, and 33.33%~61.54% of the cases adjusted their treatment regimens according to the negative mNGS results. Most patients who had their treatment adjusted showed improvement or relief of symptoms. Overall, various pathogenic microorganisms were detected in more than 60% of the samples.</p> Research conclusion <p> This study confirms the significant advantages of mNGS in the precise diagnosis of infectious diseases, as well as its value in guiding individualized treatment strategies.</p>

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Clinical utility of metagenomic next-generation sequencing in precision diagnosis of infectious diseases: a retrospective study based on bronchoalveolar lavage fluid, blood, and cerebrospinal fluid

  • 期刊论文

  • 发表日期：

    2026年5月28日

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  • Congwen Shao,
  • Jun Li,
  • Chunyan Huang,
  • Mei Tang,
  • Junzhao Zeng,
  • Wenyan Zhou,
  • Dandan Zhang,
  • Guansheng Zeng,
  • Jianwei Wang,
  • Tian Hua,
  • Chuhang Zhong,
  • Jie Hu,
  • Xueqing Xu

摘要

Research background

Metagenomic next-generation sequencing (mNGS) is a culture-independent pathogen identification method, which can directly sequence all nucleic acids present in clinical samples, and has shown transformative potential in the diagnostic field of complex, critical and emerging infectious diseases, but its clinical application value has not been fully evaluated. This study aims to compare the diagnostic efficacy of mNGS and traditional microbiological testing (TMT), and evaluate its impact on clinical decision-making.

Research methods

This retrospective study analyzed the data of the laboratory information system (LIS) of patients who received both mNGS and TMT testing.

Research results

In samples of bronchoalveolar lavage fluid (BALF), blood and cerebrospinal fluid (CSF), the positive rates of mNGS were 86.70%, 77.17% and 53.57% respectively, which were significantly higher than the corresponding positive rates of TMT (41.38%, 14.13%, 17.86%). Clinical correlation analysis showed that 77.84%, 66.20% and 73.33% of the positive mNGS results of the three types of samples were of clinical significance respectively. 15.34%~29.58% of the cases adjusted their treatment regimens according to the positive mNGS results, and 33.33%~61.54% of the cases adjusted their treatment regimens according to the negative mNGS results. Most patients who had their treatment adjusted showed improvement or relief of symptoms. Overall, various pathogenic microorganisms were detected in more than 60% of the samples.

Research conclusion

This study confirms the significant advantages of mNGS in the precise diagnosis of infectious diseases, as well as its value in guiding individualized treatment strategies.