Purpose <p>Two lateral flow immunochromatographic assays are currently available for the rapid detection of CTX-M enzymes in <i>Enterobacterales</i>, RESIST CTX-M (Coris BioConcept, Gembloux, Belgium) and NG-Test CTX-M MULTI (NG Biotech, Guipry, France). The NG-Test CTX-M MULTI is designed to detect all CTX-M enzymes. The RESIST CTX-M detects CTX-M from the M-15 and the M-14 group for V1.0, CTX-M from the M-15 and the M-14/M-8 groups for V1.1.</p> Method <p>The performances of the two versions of the RESIST CTX-M test (V1.0 and V1.1) and the NG-Test CTX-M MULTI were assessed against a collection of 140 non-redundant <i>Enterobacterales</i> isolates characterized for their content in β-lactamases. β-lactamases content was established from genotypic analysis to collect a broad diversity of resistance mechanisms and bacterial strains, including 74 ESBL-producing strains (CTX-M (<i>n</i> = 56), other ESBLs (<i>n</i> = 18) either alone or associated with other β-lactamases), 22 strains overproducing chromosomal AmpC, 13 strains producing plasmid-encoded AmpC, 7 carbapenemase-producing strains, 3 strains combining the production of several β-lactamases other than ESBL and 21 strains that produced other β-lactamases.</p> Results <p>The sensitivity and specificity of NG-Test CTX-M MULTI for detecting CTX-M were 98.2% and 85.7%, respectively. They were 87.2% and 93.5% for RESIST CTX-M V1.0 for CTX-M of the M-1 and M-9 groups versus 94.3% and 93.1% for RESIST CTX-M V1.1 for CTX-M of the M-1 and M-9/8 groups.</p> Conclusion <p>Lateral flow immunochromatographic assays are rapid and reliable tests for the detection of CTX-M production. The NG-Test CTX-M MULTI and RESIST CTX-M V1.1 were the most efficient. However, one limitation of the different assays is the risk of false-positive results with strains belonging to the <i>K. oxytoca</i> and <i>C. farmeri/sedlakii/amalonaticus</i> complexes, which overproduce their chromosomal β-lactamase.</p>