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<p>This study investigates a diagnostic challenge in detecting <i>Streptococcus pneumoniae</i>&#xa0;using a Spn9802-targeted PCR assay. Four <i>S. pneumoniae</i> isolates tested <i>lytA</i>-positive but Spn9802-negative due to an insertion sequence (IS<i>1515</i>) within Spn9802, which was confirmed by whole-genome sequencing. This insertion may lead to false-negative results, posing concerns for PCR-based diagnostics. The study emphasizes the need for dual-target PCR and regular updates to diagnostic primers, underscoring the importance of continuous assay evaluation and post-market surveillance to ensure reliable pathogen detection.</p>

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Discovery of IS1515 transposition into the Spn9802 fragment of Streptococcus pneumoniae and its implications for PCR-based diagnostic assays

  • 期刊论文

  • 发表日期：

    2025年11月2日

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  • Myrthe M.A. Toorop,
  • Margriet E.M. Kraakman,
  • Eric C.J. Claas,
  • Els Wessels,
  • Stefan A. Boers

摘要

This study investigates a diagnostic challenge in detecting Streptococcus pneumoniae using a Spn9802-targeted PCR assay. Four S. pneumoniae isolates tested lytA-positive but Spn9802-negative due to an insertion sequence (IS1515) within Spn9802, which was confirmed by whole-genome sequencing. This insertion may lead to false-negative results, posing concerns for PCR-based diagnostics. The study emphasizes the need for dual-target PCR and regular updates to diagnostic primers, underscoring the importance of continuous assay evaluation and post-market surveillance to ensure reliable pathogen detection.