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Background <p>Intraamniotic infection is a cause of spontaneous preterm labor. <i>Streptococcus mitis</i> is a common pathogen identified in intraamniotic infection, with the possible route of hematogenous dissemination from the oral cavity or migration from the vaginal canal. However, there are a few reports on <i>Streptococcus oralis</i>, a member of the <i>S. mitis</i> group, as a cause of pathogen in intraamniotic infection. We reported herein whole genome sequencing and comparative genomic analysis of <i>S. oralis</i> strain RAOG5826 that causes intraamniotic infection.</p> Results <p><i>Streptococcus mitis</i> was initially identified from amniotic fluid, vaginal swab, and fetal blood of a patient presenting with preterm prelabor rupture of membranes with intraamniotic infection by the use of conventional microbiological methods (biochemical phenotype, MALDI-ToF, 16&#xa0;S rRNA). Subsequently, this strain was later identified as <i>S. oralis</i> RAOG5826 by whole-genome hybrid sequencing. Genes involved in macrolide and tetracycline resistance, namely <i>ermB</i> and <i>tet(M)</i>, and mutations in penicillin-binding protein were present in the genome. Moreover, potential virulence genes were predicted and compared with other Streptococcal species.</p> Conclusion <p>We reported a comprehensive genomic analysis of <i>S. oralis</i>, which causes intraamniotic infection. <i>S. mitis</i> was initially identified by conventional microbiological identification. However, whole-genome hybrid sequencing demonstrates <i>S. oralis</i> with complete profiles of antimicrobial resistance genes and potential virulence factors. This study highlights the limitations of traditional techniques and underscores the importance of genomic sequencing for accurate diagnosis and tailored antimicrobial treatment. The study also suggests that <i>S. oralis</i> may be an underestimated pathogen in intraamniotic infection.</p>

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Genomic identification and characterization of Streptococcus oralis group that causes intraamniotic infection

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月29日

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  • Pisut Pongchaikul,
  • Puntabut Warintaksa,
  • Thidathip Wongsurawat,
  • Piroon Jenjaroenpun,
  • Worarat Kruasuwan,
  • Paninee Mongkolsuk,
  • Pornpun Vivithanaporn,
  • Iyarit Thaipisuttikul,
  • Arunee Singhsnaeh,
  • Jakkrit Khamphakul,
  • Suwatcharaporn Hadradchai,
  • Maolee Bhuwapathanapun,
  • Piya Chaemsaithong

摘要

Background

Intraamniotic infection is a cause of spontaneous preterm labor. Streptococcus mitis is a common pathogen identified in intraamniotic infection, with the possible route of hematogenous dissemination from the oral cavity or migration from the vaginal canal. However, there are a few reports on Streptococcus oralis, a member of the S. mitis group, as a cause of pathogen in intraamniotic infection. We reported herein whole genome sequencing and comparative genomic analysis of S. oralis strain RAOG5826 that causes intraamniotic infection.

Results

Streptococcus mitis was initially identified from amniotic fluid, vaginal swab, and fetal blood of a patient presenting with preterm prelabor rupture of membranes with intraamniotic infection by the use of conventional microbiological methods (biochemical phenotype, MALDI-ToF, 16 S rRNA). Subsequently, this strain was later identified as S. oralis RAOG5826 by whole-genome hybrid sequencing. Genes involved in macrolide and tetracycline resistance, namely ermB and tet(M), and mutations in penicillin-binding protein were present in the genome. Moreover, potential virulence genes were predicted and compared with other Streptococcal species.

Conclusion

We reported a comprehensive genomic analysis of S. oralis, which causes intraamniotic infection. S. mitis was initially identified by conventional microbiological identification. However, whole-genome hybrid sequencing demonstrates S. oralis with complete profiles of antimicrobial resistance genes and potential virulence factors. This study highlights the limitations of traditional techniques and underscores the importance of genomic sequencing for accurate diagnosis and tailored antimicrobial treatment. The study also suggests that S. oralis may be an underestimated pathogen in intraamniotic infection.