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<p><i>Staphylococcus aureus</i> blood cultures were investigated geno- and phenotypically for penicillin susceptibility. A qPCR method detecting the <i>blaZ</i> gene was performed directly on positive blood culture vials and compared with routine disk diffusion results supplemented with the cloverleaf test. Among the collected vials, 100 were <i>blaZ</i> positive and 101 were <i>blaZ</i> negative. Full concordance was observed between genotypical and phenotypical penicillin susceptibility, however one penicillin resistant isolate was only weakly phenotypically positive.</p>

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Staphylococcus aureus genotypic penicillin susceptibility using a blaZ qPCR directly on blood cultures shows high concordance with phenotype

  • 期刊论文

  • 发表日期：

    2025年9月25日

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  • Tobias Müller Vang,
  • Barbara Juliane Holzknecht,
  • Stephen Strunge Nilsson,
  • Morten Hoppe,
  • Michael Pedersen,
  • Karen Angeliki Krogfelt,
  • Helle Brander Eriksen

摘要

Staphylococcus aureus blood cultures were investigated geno- and phenotypically for penicillin susceptibility. A qPCR method detecting the blaZ gene was performed directly on positive blood culture vials and compared with routine disk diffusion results supplemented with the cloverleaf test. Among the collected vials, 100 were blaZ positive and 101 were blaZ negative. Full concordance was observed between genotypical and phenotypical penicillin susceptibility, however one penicillin resistant isolate was only weakly phenotypically positive.