Scholarlink

Background <p>We aimed to describe the genomic epidemiology of vancomycin-resistant <i>Enterococcus faecium</i> (VREfm) in Eastern Denmark from 2020 to 2022, identify and characterise the <i>vanB</i> Transposon <i>1549</i> (Tn<i>1549</i>) insertion sites among <i>vanB</i> VREfm clones and identify emerging VREfm clones.</p> Methods <p>We analysed all VREfm from our routine diagnostic sequencing during the study period. Using the Seqsphere + v.8.2.0 software (Ridom GmbH, Münster, Germany, (<a href="http://www.ridom.de/seqsphere">http://www.ridom.de/seqsphere</a>), minimum spanning trees were created to visualise clusters. Tn<i>1549</i> insertion sites were determined by in silico PCR. Nanopore sequencing was performed to assemble the regions surrounding Tn<i>1549</i>, which helped determine the insertion site locations.</p> Results <p>We included 2,437 isolates in the study. A total of 463 isolates carried <i>vanA</i>, 1,963 isolates carried <i>vanB</i>, and 11 isolates carried both genes. Of all isolates carrying <i>vanB</i>, 254 isolates had the Tn<i>1549</i> inserted in the <i>araA2</i> gene, 1,604 in the <i>sir2</i> gene, and 116 in neither the <i>araA2</i> nor <i>sir2</i> genes. We identified eight chromosomal insertion sites other than in the <i>araA2</i> and <i>sir2</i> genes. Three isolates carried the Tn<i>1549</i> on plasmids. No emerging clones were found.</p> Results <p>We have described the genomic epidemiology during the study period and identified ten chromosomal Tn<i>1549</i> insertion sites.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genomic epidemiology of vancomycin-resistant Enterococcus faecium in Eastern Denmark from 2020 to 2022, and identification of vanB Tn1549 insertion sites

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年4月1日

查看全文
  • Maja Johanne Søndergaard Knudsen,
  • Jose Alfredo Samaniego Castruita,
  • Ingrid Maria Cecilia Rubin,
  • Sarah Mollerup,
  • Helle Krogh Johansen,
  • Rasmus L. Marvig,
  • Karen Leth Nielsen,
  • Barbara Juliane Holzknecht,
  • Morten Hoppe,
  • Michael Kemp,
  • Henrik Westh,
  • Mette Pinholt

摘要

Background

We aimed to describe the genomic epidemiology of vancomycin-resistant Enterococcus faecium (VREfm) in Eastern Denmark from 2020 to 2022, identify and characterise the vanB Transposon 1549 (Tn1549) insertion sites among vanB VREfm clones and identify emerging VREfm clones.

Methods

We analysed all VREfm from our routine diagnostic sequencing during the study period. Using the Seqsphere + v.8.2.0 software (Ridom GmbH, Münster, Germany, (http://www.ridom.de/seqsphere), minimum spanning trees were created to visualise clusters. Tn1549 insertion sites were determined by in silico PCR. Nanopore sequencing was performed to assemble the regions surrounding Tn1549, which helped determine the insertion site locations.

Results

We included 2,437 isolates in the study. A total of 463 isolates carried vanA, 1,963 isolates carried vanB, and 11 isolates carried both genes. Of all isolates carrying vanB, 254 isolates had the Tn1549 inserted in the araA2 gene, 1,604 in the sir2 gene, and 116 in neither the araA2 nor sir2 genes. We identified eight chromosomal insertion sites other than in the araA2 and sir2 genes. Three isolates carried the Tn1549 on plasmids. No emerging clones were found.

Results

We have described the genomic epidemiology during the study period and identified ten chromosomal Tn1549 insertion sites.