Molecular analysis of metallo-beta-lactamase-producing Pseudomonas aeruginosa in Switzerland 2022–2023

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2024年1月18日

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Jacqueline Findlay,
Otavio Hallal Ferreira Raro,
Laurent Poirel,
Patrice Nordmann,
R. Lienhard,
L. Vonallmen,
C. Schilt,
A. Scherler,
K. Lucke,
M. Jutzi,
M. Reichmuth,
U. Schibli,
C. Fricker,
S. Pranghofer,
G. Greub,
D. Blanc,
A. Vitale,
B. Lemaire,
M. Fatoux,
M. Tritten,
L. Rumebe,
N. Liassine,
G. Jost,
N. Wohlwend,
D. Schultze,
K. Burren,
A. Westers,
M. Imperiali,
L. Pozzi,
D. Balzari,
G. Vaninetti,
C. Cirillo,
V. Gaia,
E. Pianezzi,
G. Martinetti Lucchini,
F. Baggi Menozzi,
A. Jayol,
C. Guyon,
D. Hyden,
M. Maitrejean,
V. Deggi-Messmer,
D. Bandeira,
C. Fournier,
S. Pfister,
C. Nusbaumer,
L. Bertaiola Monnerat,
J. Schrenzel,
G. Renzi,
A. Cherkaoui,
D. Andrey,
S. Emonet,
M. Eyer,
R. Maret,
A. Belo,
D. Mabillard,
M. Moraz,
K. Herzog,
V. Gisler,
E. Hitz,
M. Oberle,
C. Castelberg,
H. Fankhauser,
S. Graf,
N. Dubey,
C. Guler,
M. Schoenenberger,
U. Karrer,
F. Piran,
C. Andreutti,
M. Dessauges,
T. Schmid,
B. Suterbuser,
I. Mitrovic,
E. Gruner,
V. Bruderer,
P. Staehli,
B. Schnell,
C. O. Marti,
I. Steffen,
A. Imhof,
B. Preiswerk,
V. Dilorenzo,
C. Payen,
D. Boschung,
L. Comte,
M. Schacher,
M. Brandenberger,
C. Zowa,
C. Zehnder,
B. Mathis,
L. Basilico,
G. Togni,
P. Minkova,
Y. Born,
M. Kuegler,
V. Povolo,
S. Droz,
M. Elzi,
C. Casanova,
D. Goldenberger,
P. Keller,
C. Lang,
A. Blaich,
S. Schmid,
B. Ivan,
A. Egli,
S. Mancini,
O. Dubois,
K. Narr,
S. Schoch,
S. Ellenberger,
S. Seiffert

摘要

Objectives

The occurrence of metallo-beta-lactamase-producing Pseudomonas aeruginosa (MBL-PA) isolates is increasing globally, including in Switzerland. The aim of this study was to characterise, phenotypically and genotypically, the MBL-PA isolates submitted to the Swiss National Reference Center for Emerging Antibiotic Resistance (NARA) reference laboratory over a 12-month period from July 2022 to July 2023.

Methods

Thirty-nine non-duplicate MBL-PA Isolates were submitted to NARA over the study period from across Switzerland. Susceptibility was determined by broth microdilution according to EUCAST methodology. Whole-genome sequencing was performed on 34 isolates. Sequence types (STs) and resistance genes were ascertained using the Centre for Genomic Epidemiology platform. MBL genes, bla_NDM-1, bla_IMP-1, and bla_VIM-2, were cloned into vector pUCP24 and transformed into P. aeruginosa PA14.

Results

The most prevalent MBL types identified in this study were VIM (21/39; 53.8%) followed by NDM (11/39; 28.2%), IMP (6/39; 15.4%), and a single isolate produced both VIM and NDM enzymes. WGS identified 13 different STs types among the 39 isolates. They all exhibited resistance to cephalosporins, carbapenems, and the beta-lactam-beta-lactamase inhibitor combinations, ceftolozane-tazobactam, ceftazidime-avibactam, imipenem-relebactam, and meropenem-vaborbactam, and 8 isolates were cefiderocol (FDC) resistant. Recombinant P. aeruginosa strains producing bla_NDM-1, bla_IMP-1, and bla_VIM-2 exhibited FDC MICs of 16, 8, and 1 mg/L, respectively.

Conclusions

This study showed that the MBL-PA in Switzerland could be attributed to the wide dissemination of high-risk clones that accounted for most isolates in this study. Although FDC resistance was only found in 8 isolates, MBL carriage was shown to be a major contributor to this phenotype.