Differences in PPD- and mitogen-induced T-cell activation marker expression characterize immunopathology in acute tuberculosis patients

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2024年1月3日

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Isaac Acheampong,
Difery Minadzi,
Edwin F. Laing,
Michael Frimpong,
Monika M. Vivekanandan,
Augustine Yeboah,
Ernest Adankwah,
Wilfred Aniagyei,
Joseph F. Arthur,
Millicent Lamptey,
Mohammed K. Abass,
Francis Kumbel,
Francis Osei-Yeboah,
Amidu Gawusu,
Linda Batsa Debrah,
Dorcas O. Owusu,
Alexander Debrah,
Ertan Mayatepek,
Julia Seyfarth,
Richard O. Phillips,
Marc Jacobsen

摘要

Impaired T-cell responses to mitogens and high T-cell activation marker (TAM) expression on Mycobacterium tuberculosis–specific T-cells characterize immunopathology in patients with tuberculosis (TB). In a study of patients with TB (n = 60) and asymptomatic contacts (controls, n = 37), we found that TB patients had higher CD38⁺ T-cell proportions specific for M. tuberculosis protein (PPD_Mtb), yet total proportions of PPD_Mtb-specific T-cells were comparable. Notably, both activated (CD38⁺) and total IFN-γ⁺ T-cells from TB patients had lower mitogen (phytohemagglutinin, PHA)-induced responses. This impaired mitogen response improved the classification efficacy of the TAM-TB assay, especially employing the PPD/PHA-induced T-cell ratio.