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<p>Crude lipids from boiled ark shell (<i>Scapharca subcrenata</i>) were extracted using either Folch solvent (chloroform:methanol, 2:1, v/v) at room temperature or 95% ethanol under heating conditions. After phospholipase A<sub>1</sub> (PLA<sub>1</sub>) treatment, lipids were recovered using either Folch solvent or HIP solvent (hexane:isopropanol, 3:2, v/v). Accordingly, three experimental conditions were established based on the extraction and recovery solvent combinations, namely F-F, E-F, and E-HIP. Highest lipid recovery rate (71.1%) was observed under the E-HIP, and HPLC-ELSD analysis revealed that the ethanol extracts (E-F: 24.4&#xa0;mg/g, E-HIP: 28.2&#xa0;mg/g) exhibited higher ether-linked PC contents than the Folch extract (F-F: 15.2&#xa0;mg/g). GC-FID analysis of the sn-2 fatty acid composition of ether-linked PC showed that unsaturated fatty acids accounted for more than 70% in all samples. LC-ESI-MS analysis revealed a dominant ion at <i>m/z</i> 792 in all samples, corresponding to ether-linked PC species containing a polyunsaturated fatty acid at the sn-2 position.</p>

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Characterization of ether-linked phosphatidylcholine from boiled ark shell (Scapharca subcrenata) lipids using different extraction and recovery solvent systems

  • 期刊论文

  • 发表日期：

    2026年5月28日

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  • Ga-Eun Choi,
  • Hae-Se Lee,
  • Min-Jeong Kim,
  • Sung-Kwang Lee,
  • Ki-Teak Lee

摘要

Crude lipids from boiled ark shell (Scapharca subcrenata) were extracted using either Folch solvent (chloroform:methanol, 2:1, v/v) at room temperature or 95% ethanol under heating conditions. After phospholipase A1 (PLA1) treatment, lipids were recovered using either Folch solvent or HIP solvent (hexane:isopropanol, 3:2, v/v). Accordingly, three experimental conditions were established based on the extraction and recovery solvent combinations, namely F-F, E-F, and E-HIP. Highest lipid recovery rate (71.1%) was observed under the E-HIP, and HPLC-ELSD analysis revealed that the ethanol extracts (E-F: 24.4 mg/g, E-HIP: 28.2 mg/g) exhibited higher ether-linked PC contents than the Folch extract (F-F: 15.2 mg/g). GC-FID analysis of the sn-2 fatty acid composition of ether-linked PC showed that unsaturated fatty acids accounted for more than 70% in all samples. LC-ESI-MS analysis revealed a dominant ion at m/z 792 in all samples, corresponding to ether-linked PC species containing a polyunsaturated fatty acid at the sn-2 position.