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<p>The global seaweed industry has expanded rapidly owing to increasing demand for sustainable food resources and bioactive compounds. <i>Undaria pinnatifida</i>, a widely cultivated brown seaweed in East Asia, possesses nutritional and medicinal properties; however, it is often misidentified as the morphologically similar non-commercial brown alga <i>Endarachne binghamiae</i>, leading to accidental admixture during harvesting and processing. Herein, we developed a SYBR Green-based quantitative PCR (qPCR) assay targeting species-specific single-nucleotide polymorphisms and insertions/deletions to accurately distinguish the two species. Six primer sets were designed and validated, yielding correlation coefficients over 0.99, amplification slopes of − 3.23 to − 3.43, and efficiencies of 95.41%–103.98%. The primers exhibited high specificity without false-positive reactions when tested against two target and 13 non-target seaweed species. The assay was successfully applied to 15 commercial <i>U. pinnatifida</i> products. This qPCR assay is a rapid, sensitive, and cost-effective tool for authenticating similar seaweed species, ensuring food safety and quality.</p>

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Development of a SYBR Green-based quantitative PCR assay for the detection of Undaria pinnatifida and Endarachne binghamiae using chloroplast markers in processed seaweed products

  • 期刊论文

  • 发表日期：

    2026年4月25日

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  • Su Yeon Kim,
  • Ju Hee Kim,
  • Cheol Seong Jang

摘要

The global seaweed industry has expanded rapidly owing to increasing demand for sustainable food resources and bioactive compounds. Undaria pinnatifida, a widely cultivated brown seaweed in East Asia, possesses nutritional and medicinal properties; however, it is often misidentified as the morphologically similar non-commercial brown alga Endarachne binghamiae, leading to accidental admixture during harvesting and processing. Herein, we developed a SYBR Green-based quantitative PCR (qPCR) assay targeting species-specific single-nucleotide polymorphisms and insertions/deletions to accurately distinguish the two species. Six primer sets were designed and validated, yielding correlation coefficients over 0.99, amplification slopes of − 3.23 to − 3.43, and efficiencies of 95.41%–103.98%. The primers exhibited high specificity without false-positive reactions when tested against two target and 13 non-target seaweed species. The assay was successfully applied to 15 commercial U. pinnatifida products. This qPCR assay is a rapid, sensitive, and cost-effective tool for authenticating similar seaweed species, ensuring food safety and quality.