Scholarlink

<p>A synergistic enzymatic–ethanolic extraction process was developed to recover arbutin, a whitening and anti-wrinkle bioactive compound, from boiled pear pomace, in which boiling loosened the fiber network. To overcome the low yield and high energy consumption of conventional high-temperature and supercritical extraction methods, this study combined Alcalase 2.4L FG hydrolysis with 80% ethanol extraction. Optimal enzymatic conditions (0.024 U enzyme, 10% substrate, 24&#xa0;h), under which arbutin yield reached 9.40&#xa0;mg/g and further increased to 10.87&#xa0;mg/g following ethanolic extraction. Compared with the α-MSH–stimulated group, the synergistic extract significantly inhibited tyrosinase activity by 68% and melanin production by 63% in B16F10 melanoma cells and reduced UVB-induced MMP-1 by 82% while enhancing procollagen I α1 (4.43-fold) and hyaluronic acid (3.65-fold) in dermal fibroblasts. Correlation analysis confirmed that arbutin content aligned with whitening and anti-aging markers. This synergistic extraction process demonstrates potential for valorizing pear by-products into multifunctional cosmetic ingredients.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Synergistic enzymatic − ethanol extraction of arbutin from boiled pear pomace and its whitening and anti-wrinkle activities

  • 期刊论文

  • 发表日期：

    2026年3月27日

查看全文
  • Hyeon-Jun Seong,
  • Yunha Bak,
  • Sumin Pyeon,
  • Sock Gon Jeong,
  • Kwang-Yeol Yang,
  • Seung-Hee Nam

摘要

A synergistic enzymatic–ethanolic extraction process was developed to recover arbutin, a whitening and anti-wrinkle bioactive compound, from boiled pear pomace, in which boiling loosened the fiber network. To overcome the low yield and high energy consumption of conventional high-temperature and supercritical extraction methods, this study combined Alcalase 2.4L FG hydrolysis with 80% ethanol extraction. Optimal enzymatic conditions (0.024 U enzyme, 10% substrate, 24 h), under which arbutin yield reached 9.40 mg/g and further increased to 10.87 mg/g following ethanolic extraction. Compared with the α-MSH–stimulated group, the synergistic extract significantly inhibited tyrosinase activity by 68% and melanin production by 63% in B16F10 melanoma cells and reduced UVB-induced MMP-1 by 82% while enhancing procollagen I α1 (4.43-fold) and hyaluronic acid (3.65-fold) in dermal fibroblasts. Correlation analysis confirmed that arbutin content aligned with whitening and anti-aging markers. This synergistic extraction process demonstrates potential for valorizing pear by-products into multifunctional cosmetic ingredients.