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<p>Probiotics are live microorganisms that confer health benefits to the host through adequate viability, safety, and functional interaction with host cells. In this study, <i>Bifidobacterium longum</i> subsp. <i>infantis</i> JNU311 (JNU311) was isolated from the feces of a 3-week-old newborn, and its probiotic potential was assessed. Among the tested <i>Bifidobacterium</i> strains, JNU311 achieved the highest viable cell count (1.5 × 10<sup>9</sup>&#xa0;CFU/mL) after 36&#xa0;h in 10% skim milk, indicating suitability for fermented dairy applications. JNU311 did not inhibit growth of <i>Lactobacillus acidophilus</i> or <i>Streptococcus thermophilus</i> in mixed cultures. Functional characterization revealed that JNU311 was nonhemolytic and tolerant to acidic and bile conditions. Moreover, it exhibited bile salt hydrolase activity. The strain effectively adhered to HT-29 intestinal epithelial cells, showed no cytotoxicity in MTT assays, met antibiotic safety criteria, and reduced TNF-α production in LPS-stimulated RAW 264.7 cells. These findings support probiotic potential of JNU311 for use in health-promoting dairy products.</p>

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Characterization and probiotic potential of Bifidobacterium longum subsp. infantis JNU311 isolated from infant feces

  • 期刊论文

  • 发表日期：

    2026年3月9日

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  • Subin Kim,
  • Kiyeop Kim,
  • Ziyao Meng,
  • Seungji Kang,
  • Sejong Oh

摘要

Probiotics are live microorganisms that confer health benefits to the host through adequate viability, safety, and functional interaction with host cells. In this study, Bifidobacterium longum subsp. infantis JNU311 (JNU311) was isolated from the feces of a 3-week-old newborn, and its probiotic potential was assessed. Among the tested Bifidobacterium strains, JNU311 achieved the highest viable cell count (1.5 × 109 CFU/mL) after 36 h in 10% skim milk, indicating suitability for fermented dairy applications. JNU311 did not inhibit growth of Lactobacillus acidophilus or Streptococcus thermophilus in mixed cultures. Functional characterization revealed that JNU311 was nonhemolytic and tolerant to acidic and bile conditions. Moreover, it exhibited bile salt hydrolase activity. The strain effectively adhered to HT-29 intestinal epithelial cells, showed no cytotoxicity in MTT assays, met antibiotic safety criteria, and reduced TNF-α production in LPS-stimulated RAW 264.7 cells. These findings support probiotic potential of JNU311 for use in health-promoting dairy products.