<p>This study investigated the role of the degree of polymerization (DP) and the inclusion level of cellulose in governing the anisotropy and texture of soy protein isolate-based high-moisture meat analogs. Low-DP cellulose offered modest reinforcement, leading to a slight increase in hardness and limited enhancement of fibrous alignment. In contrast, high-DP cellulose markedly increased hardness and chewiness with increasing loading, while springiness and cohesiveness were unaffected. At 3% high-DP cellulose, the texturization index peaked with highly aligned lamellae, whereas 5% further stiffened the network but reduced anisotropy due to over-consolidation. Fourier transform infrared spectroscopy confirmed a transition from coil/helix to β-sheet structures, peaking at 3% high-DP cellulose. Chemical probing revealed progressive gains in hydrogen bonding and hydrophobic interactions, with disulfide bonds exhibiting a maximum under the same condition. Overall, cellulose chain length dictates function: low-DP densifies, and high-DP aligns and reinforces, with ≈3% high-DP cellulose optimal for anisotropic fibrous networks.</p>

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Role of degree of polymerization of cellulose in governing anisotropy and texture of high-moisture meat analogs

  • Hyun Woo Choi,
  • Heeseo Lee,
  • Hyung Joo Kim,
  • Jungwoo Hahn,
  • Young Jin Choi

摘要

This study investigated the role of the degree of polymerization (DP) and the inclusion level of cellulose in governing the anisotropy and texture of soy protein isolate-based high-moisture meat analogs. Low-DP cellulose offered modest reinforcement, leading to a slight increase in hardness and limited enhancement of fibrous alignment. In contrast, high-DP cellulose markedly increased hardness and chewiness with increasing loading, while springiness and cohesiveness were unaffected. At 3% high-DP cellulose, the texturization index peaked with highly aligned lamellae, whereas 5% further stiffened the network but reduced anisotropy due to over-consolidation. Fourier transform infrared spectroscopy confirmed a transition from coil/helix to β-sheet structures, peaking at 3% high-DP cellulose. Chemical probing revealed progressive gains in hydrogen bonding and hydrophobic interactions, with disulfide bonds exhibiting a maximum under the same condition. Overall, cellulose chain length dictates function: low-DP densifies, and high-DP aligns and reinforces, with ≈3% high-DP cellulose optimal for anisotropic fibrous networks.