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<p>A magnetic extraction/recovery platform for carotenoids was developed using casein-functionalized, chitosan-coated MnFe₂O₄ nanoparticles (Cas@CSMNP). Nanoparticles were prepared by coprecipitation followed by hydrothermal treatment and characterized by XRD, FTIR, DLS, TGA, TEM, and magnetometry, confirming a spinel core and successful casein coating. A design-of-experiments approach identified conditions that maximized casein immobilization (80&#xa0;mg&#xa0;g⁻<sup>1</sup> at 40&#xa0;°C) consistent with favorable kinetic/isotherm fits. The results of assays with β-carotene standards and <i>Rhodotorula toruloides</i> extracts showed rapid uptake, with 73.1% and 84.2% adsorption, respectively, after 1&#xa0;h at 30&#xa0;°C. Cell-viability assays (1132 SK, RAW 264.7) indicated &gt; 90% viability at 50–100&#xa0;µg&#xa0;mL⁻<sup>1</sup>. Cas@CSMNP integrates protein affinity with magnetic separability under ethanol-compatible conditions, providing a practical, potentially solvent-saving route for selective recovery of carotenoids from yeast extracts and a foundation for future evaluation of desorption and reuse.</p> Graphical Abstract <p></p>

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Casein-functionalized manganese ferrite nanostructures for selective extraction of carotenoids from Rhodotorula toruloides

  • 期刊论文

  • 发表日期：

    2026年1月18日

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  • Nayra Ochoa-Viñals,
  • Dania Alonso-Estrada,
  • Ariel García-Cruz,
  • Rodolfo Ramos-González,
  • Enrique Díaz Barriga-Castro,
  • Evelyn Faife-Perez,
  • Georgina Michelena-Álvarez,
  • Rebeca Betancourt-Galindo,
  • Anna Ilina

摘要

A magnetic extraction/recovery platform for carotenoids was developed using casein-functionalized, chitosan-coated MnFe₂O₄ nanoparticles (Cas@CSMNP). Nanoparticles were prepared by coprecipitation followed by hydrothermal treatment and characterized by XRD, FTIR, DLS, TGA, TEM, and magnetometry, confirming a spinel core and successful casein coating. A design-of-experiments approach identified conditions that maximized casein immobilization (80 mg g⁻1 at 40 °C) consistent with favorable kinetic/isotherm fits. The results of assays with β-carotene standards and Rhodotorula toruloides extracts showed rapid uptake, with 73.1% and 84.2% adsorption, respectively, after 1 h at 30 °C. Cell-viability assays (1132 SK, RAW 264.7) indicated > 90% viability at 50–100 µg mL⁻1. Cas@CSMNP integrates protein affinity with magnetic separability under ethanol-compatible conditions, providing a practical, potentially solvent-saving route for selective recovery of carotenoids from yeast extracts and a foundation for future evaluation of desorption and reuse.

Graphical Abstract