Attenuation of IL-1β-induced inflammation in intestinal epithelial cells by Centella asiatica juice via MAPK and NF-κB signaling cascades and characterization of its key bioactive compound
摘要
Centella asiatica (CA) has served as a cornerstone of Ayurvedic and other traditional therapies for inflammatory disorders for decades. Although the anti-inflammatory properties of CA extracts have been increasingly recognized, the impact of CA juice (CA-Juice) on intestinal inflammation remains largely unexplored. This study explored the functional properties of CA-Juice in an in vitro model of intestinal inflammation and identified a key bioactive compound. CA-Juice significantly attenuated the secretion and gene expression of pro-inflammatory mediators. It not only upregulated tight junction-related proteins but also increased the expression of their corresponding genes. The observed effects were largely attributable to the modulation of key inflammatory signaling cascades, including those related to MAPKs and NF-κB. UHPLC-MS2 revealed miquelianin as the dominant constituent and functional mediator. The results highlight CA-Juice as a promising functional approach for mitigating intestinal inflammation and offer insight into its potential as an alternative therapeutic option.