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<p>This study aimed to develop a non-genetically modified organism (non-GMO) <i>Saccharomyces cerevisiae</i> strain with enhanced glutathione production using UV-induced random mutagenesis. Among 30 wild-type strains, <i>S. cerevisiae</i> KACC 48331 was selected for its high glutathione production. A #14 mutant with significantly higher glutathione content was isolated via acrolein resistance screening. Optimal batch fermentation was established at 20&#xa0;°C and pH 4.5 using a cost-effective medium of molasses and corn steep liquor (CSL). In fed-batch fermentation, the #14 mutant achieved 1.98&#xa0;g/L of glutathione using molasses and CSL as carbon and nitrogen sources, comparable to yields from expensive complex media containing peptone and yeast extract. Notably, over 99% of the glutathione produced was in the reduced, biologically active form. These results demonstrate the feasibility of using non-GMO yeast and industrial by-products for sustainable and economical glutathione production.</p>

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Enhanced glutathione production by a non-GMO Saccharomyces cerevisiae mutant isolated via acrolein resistance-mediated screening

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月12日

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  • Soo Bin Nho,
  • Sang-Hun Do,
  • Seongjin Oh,
  • Yong-Cheol Park,
  • Sun-Ki Kim

摘要

This study aimed to develop a non-genetically modified organism (non-GMO) Saccharomyces cerevisiae strain with enhanced glutathione production using UV-induced random mutagenesis. Among 30 wild-type strains, S. cerevisiae KACC 48331 was selected for its high glutathione production. A #14 mutant with significantly higher glutathione content was isolated via acrolein resistance screening. Optimal batch fermentation was established at 20 °C and pH 4.5 using a cost-effective medium of molasses and corn steep liquor (CSL). In fed-batch fermentation, the #14 mutant achieved 1.98 g/L of glutathione using molasses and CSL as carbon and nitrogen sources, comparable to yields from expensive complex media containing peptone and yeast extract. Notably, over 99% of the glutathione produced was in the reduced, biologically active form. These results demonstrate the feasibility of using non-GMO yeast and industrial by-products for sustainable and economical glutathione production.