Scholarlink

<p>Intestinal parasitic diseases affected humans occur worldwide. In the Republic of Korea, <i>Clonorchis sinensis</i> and <i>Gymnophalloides seoi</i>, which comes from infected seafood, is also commonly found in the southern coastal area and causes gastroenteritis. Therefore, the aim of this study was to optimize a qPCR assay to detect and identify <i>C. sinensis</i> and <i>G. seoi</i>. After selecting a primer set for parasite detection based on literature searches, its detection limit and specificity were determined. The developed qPCR method was sensitive, specific and could be used to detect and distinguish parasites. 11 parasites were used to verify its specificity. The limit of detection and Ct values were 4.45 × 10<sup>6</sup> copy/µL and 21.024 and 3.60 × 10<sup>5</sup> copy/µL and 21.403 for <i>C. sinensis</i> and <i>G. seoi</i>, respectively. The optimized qPCR method developed herein for <i>C. sinensis</i> and <i>G. seoi</i> detection could contribute to monitoring the occurrence of these parasites in samples.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

High sensitivity detection of Clonorchis sinensis and Gymnophalloides seoi in food by new real-time gene amplification method

  • 期刊论文

  • 发表日期：

    2025年7月2日

查看全文
  • Min Ji Hong,
  • Mi-Gyeong Kim,
  • Doo Won Seo,
  • Hyun Mi Jung,
  • Hyun-Ja Han,
  • Seung Hwan Kim,
  • Insun Joo

摘要

Intestinal parasitic diseases affected humans occur worldwide. In the Republic of Korea, Clonorchis sinensis and Gymnophalloides seoi, which comes from infected seafood, is also commonly found in the southern coastal area and causes gastroenteritis. Therefore, the aim of this study was to optimize a qPCR assay to detect and identify C. sinensis and G. seoi. After selecting a primer set for parasite detection based on literature searches, its detection limit and specificity were determined. The developed qPCR method was sensitive, specific and could be used to detect and distinguish parasites. 11 parasites were used to verify its specificity. The limit of detection and Ct values were 4.45 × 106 copy/µL and 21.024 and 3.60 × 105 copy/µL and 21.403 for C. sinensis and G. seoi, respectively. The optimized qPCR method developed herein for C. sinensis and G. seoi detection could contribute to monitoring the occurrence of these parasites in samples.