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<p>Yuzu (<i>Citrus junos</i>) seeds, a by-product of juice processing, are a valuable source of oil and bioactive compounds. This study investigates the effects of air drying (40&#xa0;°C and 60&#xa0;°C), and freeze-drying methods for yuzu seeds on the physicochemical and functional properties of mechanically extracted yuzu seed oil. 40&#xa0;°C air drying was most effective, yielding the highest oil (11.43%) and vitamin E (19.38&#xa0;mg/100&#xa0;g) content, and lowest acid value (0.84–1.26&#xa0;mg KOH/g) indicating reduced rancidity. It showed greater oxidative stability, with a 1.7-fold lower peroxide value, and retention of linoleic and linolenic acids (~ 76% unsaturated fatty acids). 40&#xa0;°C air drying preserved the highest levels of anti-inflammatory compounds limonin (61&#xa0;mg/100&#xa0;g) and nomilin (32&#xa0;mg/100&#xa0;g), while 60&#xa0;°C air drying reduced them by 25% from control. In lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages, 40&#xa0;°C air-dried oil showed strongest anti-inflammatory activity, reducing nitric oxide, IL-6, and TNF-α by 1.6-, 1.43-, and 1.79-fold, respectively.</p>

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Evaluation of quality, composition and anti-inflammatory effects of yuzu seed oil obtained by different drying pretreatments

  • 期刊论文

  • 发表日期：

    2025年7月1日

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  • Bo-Bae Lee,
  • Hyeon-Ju Jeong,
  • Chang-Yong Yoon,
  • Seung-Hee Nam

摘要

Yuzu (Citrus junos) seeds, a by-product of juice processing, are a valuable source of oil and bioactive compounds. This study investigates the effects of air drying (40 °C and 60 °C), and freeze-drying methods for yuzu seeds on the physicochemical and functional properties of mechanically extracted yuzu seed oil. 40 °C air drying was most effective, yielding the highest oil (11.43%) and vitamin E (19.38 mg/100 g) content, and lowest acid value (0.84–1.26 mg KOH/g) indicating reduced rancidity. It showed greater oxidative stability, with a 1.7-fold lower peroxide value, and retention of linoleic and linolenic acids (~ 76% unsaturated fatty acids). 40 °C air drying preserved the highest levels of anti-inflammatory compounds limonin (61 mg/100 g) and nomilin (32 mg/100 g), while 60 °C air drying reduced them by 25% from control. In lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages, 40 °C air-dried oil showed strongest anti-inflammatory activity, reducing nitric oxide, IL-6, and TNF-α by 1.6-, 1.43-, and 1.79-fold, respectively.