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<p>Fucoidan has strong antioxidant and free radical scavenging activities. We successfully isolated the active fucoidan, JHCF4, from edible <i>Hizikia fusiformis</i>. The effects of high-temperature water-extracted (T-JHCF4) and cellulase-assisted extraction (C-JHCF4) of JHCF4 were compared and to demonstrate those potential for treating alcoholic liver disease&#xa0;(ALD). C-JHCF4 contained higher amounts of fucose, arabinose, rhamnose, mannose, and sulfate groups, whereas T-JHCF4 had a higher galactose content. Importantly, C-JHCF4 downregulated the expression of Bax, Caspase 3, and cytochrome C in alcohol-treated LO2 cells and upregulated Bcl-2 expression, indicating its protective effect via an apoptosis-related pathway. <i>In vivo</i>, C-JHCF4 protected alcohol-induced zebrafish from reduced reactive oxygen species production and cell death, further regulating malondialdehyde and glutathione levels. Collectively, T-JHCF4 has advantages in extraction, such as short extraction time and low cost, making it suitable for large-scale industrial production. C-JHCF4 exhibited a stronger hepatoprotective effect than T-JHCF4, indicating its suitability for treating ALD.</p>

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Fucoidan JHCF4s from Hizikia fusiformis against ethanol-induced damage in vitro and in vivo

  • 期刊论文

  • 发表日期：

    2025年4月29日

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  • Tongchuan Wu,
  • Yichao Liu,
  • Xindan Ai,
  • Sixuan Wang,
  • Aixia Hou,
  • Fei Zheng,
  • Hao Yue,
  • Yulin Dai

摘要

Fucoidan has strong antioxidant and free radical scavenging activities. We successfully isolated the active fucoidan, JHCF4, from edible Hizikia fusiformis. The effects of high-temperature water-extracted (T-JHCF4) and cellulase-assisted extraction (C-JHCF4) of JHCF4 were compared and to demonstrate those potential for treating alcoholic liver disease (ALD). C-JHCF4 contained higher amounts of fucose, arabinose, rhamnose, mannose, and sulfate groups, whereas T-JHCF4 had a higher galactose content. Importantly, C-JHCF4 downregulated the expression of Bax, Caspase 3, and cytochrome C in alcohol-treated LO2 cells and upregulated Bcl-2 expression, indicating its protective effect via an apoptosis-related pathway. In vivo, C-JHCF4 protected alcohol-induced zebrafish from reduced reactive oxygen species production and cell death, further regulating malondialdehyde and glutathione levels. Collectively, T-JHCF4 has advantages in extraction, such as short extraction time and low cost, making it suitable for large-scale industrial production. C-JHCF4 exhibited a stronger hepatoprotective effect than T-JHCF4, indicating its suitability for treating ALD.