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<p>The issue of Cervical cancer has received considerable critical attention. It was the leading cause of death in female cancer patients. Ginsenoside Rg5 has been implicated in the growth of human cancer cells, but the lack of research to determine the influence mechanism has been a significant challenge for many years. In this study, ginsenoside Rg5 was used to treat HeLa cells, and RT-qPCR, Western Blotting and RNA-seq were used to analyze the differentially expressed genes in HeLa cells to study the preliminary mechanism of ginsenoside Rg5 on cervical cancer. The results of RT-qPCR and Western Blotting were in agreement with RNA-seq analysis, which showed a down-regulation of proteins in the cell cycle pathway. These results all indicate that the expression of Minichromosome Maintenance Complex Component 6 (MCM6), Cyclin A2 (CCNA2), Cyclin-dependent kinase 6 (CDK6) and Cell division cycle 6 (CDC6) in HeLa cells treated with ginsenoside Rg5 is significantly reduced, with MCM6 exhibiting the most significant inhibitory effect, making it a potential target for the treatment of cervical cancer.</p>

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Ginsenoside Rg5 inhibits the proliferation of HeLa cell through cell cycle pathway

  • 期刊论文

  • 发表日期：

    2025年2月14日

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  • Qianwen Mi,
  • Hang Sun,
  • Heyu Wang,
  • Qiuyang Li,
  • Pei Yin,
  • Qiannan Wang,
  • Jian Tong,
  • Shuang Liu,
  • Yunfeng Bi,
  • Lei Yu

摘要

The issue of Cervical cancer has received considerable critical attention. It was the leading cause of death in female cancer patients. Ginsenoside Rg5 has been implicated in the growth of human cancer cells, but the lack of research to determine the influence mechanism has been a significant challenge for many years. In this study, ginsenoside Rg5 was used to treat HeLa cells, and RT-qPCR, Western Blotting and RNA-seq were used to analyze the differentially expressed genes in HeLa cells to study the preliminary mechanism of ginsenoside Rg5 on cervical cancer. The results of RT-qPCR and Western Blotting were in agreement with RNA-seq analysis, which showed a down-regulation of proteins in the cell cycle pathway. These results all indicate that the expression of Minichromosome Maintenance Complex Component 6 (MCM6), Cyclin A2 (CCNA2), Cyclin-dependent kinase 6 (CDK6) and Cell division cycle 6 (CDC6) in HeLa cells treated with ginsenoside Rg5 is significantly reduced, with MCM6 exhibiting the most significant inhibitory effect, making it a potential target for the treatment of cervical cancer.