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Effects of incorporated collagen/prebiotics and different coating substances on the survival rate of encapsulated probiotics

  • Sriwiang Rittisak,
  • Ratchanee Charoen,
  • Saowalak Seamsin,
  • Jamin Buppha,
  • Thanida Ruangthong,
  • Sarinya Muenkhling,
  • Wiboon Riansa-ngawong,
  • Sunee Eadmusik,
  • Kriangkrai Phattayakorn,
  • Sakwiboon Jantrasee,
  • Wanticha Savedboworn

摘要

The purpose of this study was to assess the influence of collagen protein and various prebiotics as encapsulant materials, as well as double-layer coatings composed of chitosan or sericin, on the viability of encapsulated probiotic Lactobacillus plantarum TISTR 2075. The storage stability of encapsulated probiotic was evaluated throughout 4 and 30 °C for 105 and 63 days, respectively. The log-linear inactivation rate (k) models indicated that co-encapsulated with alginate-collagen-inulin and double-layer coated with chitosan (ACI-C) had the lowest k value. Furthermore, ACI-C demonstrated the greatest value in terms of the time required for the first decimal decrease (δ) applied by Weibull non-linear model during storage at 4 and 30 °C. Encapsulated probiotic beads with ACI-C also exhibited the highest survivability after exposure to simulated gastrointestinal tract tolerance, heat resistance, and the degree hydrophobicity. The viability of cells significantly enhanced through coating with chitosan rather than sericin when employing the identical encapsulating agent.