Extraction, analysis and antioxidant activity of palm peduncle polysaccharide

The structural and antioxidant activities of palm peduncle polysaccharide was characterized, and its protect effect on hydrogen peroxide induced oxidative stress damaged for RAW264.7 cells was evaluated. The result of structural analysis showed that palm peduncle polysaccharide was main composed of rhamnoose, arabose, galactose and glucose, which accounted for 10.87%, 21.35%, 18.33% and 18.07%, respectively. The result of antioxidant activities in vitro showed that palm peduncle polysaccharide could significant increased contents of CAT, SOD, GSH-Px in superneant for RAW264.7 cells induced by H₂O₂, while the contents of MDA was decreased, showing a dose–effect relationship. In vivo activity test, palm peduncle polysaccharide could significant increased of SOD, GSH-Px activity in serum and liver of mice, and significant reduced of MDA content. The result showed that palm peduncle polysaccharide could significant increased the contents or gene expression of Nrf2, HO-1, NQO1 proteins relate to oxidative stress pathway in RAW264.7 cells damaged by H₂O₂.