Anti-obesogenic effect of standardized Brassica juncea extract on bisphenol A-induced 3T3-L1 preadipocytes and C57BL/6J obese mice

Bisphenol A (BPA) is a representative obesogen that induces adipocyte differentiation and lipid accumulation by mimicking the action of hormones. Brassica juncea is a plant belonging to the mustard family, and has antioxidant, anti-inflammatory, and anti-obesity effects. However, its efficacy against obesogen-induced obesity requires further investigation. In this study, we investigated the anti-obesogenic effects of Brassica juncea extract (BJE) on BPA-treated 3T3-L1 preadipocytes and C57BL/6J mice. The treatment with BPA and BJE did not have cytotoxic effects in vitro. In addition, BJE inhibited BPA-induced lipid accumulation and suppressed BPA-induced changes in adipocyte differentiation, adipogenesis, and lipolysis protein expression in 3T3-L1 cells. Oral administration of BJE reduced body weight, adipose tissue mass, and adipocyte size in BPA-induced obese C57BL/6 mice. BJE also regulated the expression of proteins involved in adipocyte differentiation, adipogenesis, and lipolysis. These data demonstrate that BJE is a natural functional substance with anti-obesogenic effects.