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<p>Two extremely halophilic archaeal strains, HT40<sup>T</sup> and LN261<sup>T</sup>, were isolated from salt crystal samples collected from the Dingyuan Salt Mine in Anhui, China. Strain HT40<sup>T</sup> was found to possess two copies of the 16&#xa0;S rRNA gene (<i>rrnA</i> and <i>rrnB</i>), while only one copy was detected in strain LN261<sup>T</sup>. Phylogenetic analysis based on the 16&#xa0;S rRNA gene sequences revealed that the <i>rrnA</i> and <i>rrnB</i> genes of HT40<sup>T</sup> showed high sequence similarities with <i>Halovenus aranensis</i> EB27<sup>T</sup> (94.73%) and <i>Halovenus carboxidivorans</i> WSH3<sup>T</sup> (97.42%), respectively. Strain LN261<sup>T</sup> was clustered with <i>Natronomonas aquatica</i> F2–12<sup>T</sup>, exhibiting 98.50% sequence similarity. Comparative genomic analysis demonstrated that the average nucleotide identity (ANI), digital DNA–DNA hybridization (dDDH), and average amino acid identity (AAI) values between HT40<sup>T</sup> and current species of the genus <i>Halovenus</i> ranged from 70.2 to 81.8%, 19.4–25.6%, and 62.2–83.8%, respectively. Similarly, the corresponding values between LN261<sup>T</sup> and current species of the genus <i>Natronomonas</i> were determined to be 75.0–87.3%, 21.2–38.8%, and 67.7–86.4%, respectively. All these values were significantly lower than the recommended thresholds for prokaryotic species delineation. Physiological and biochemical characterization indicated that the optimal growth conditions for HT40<sup>T</sup> were observed at 42&#xa0;°C, 4.5&#xa0;M NaCl, and pH 8.0, whereas LN261<sup>T</sup> exhibited optimal growth at 37&#xa0;°C, 3.9&#xa0;M NaCl, and pH 7.5. Based on the distinct phenotypic and genomic differences from the relevant species, strains HT40<sup>T</sup> (= MCCC 4K00167<sup>T</sup> = KCTC 4325<sup>T</sup>) and LN261<sup>T</sup> (= MCCC 4K00169<sup>T</sup> = CGMCC 1.17294<sup>T</sup> = KCTC 4321<sup>T</sup>) were proposed to represent novel species within the genera <i>Halovenus</i> and <i>Natronomonas</i>, respectively, for which the name <i>Halovenus anhuiensis</i> and <i>Natronomonas salinimetallica</i> are proposed.</p>

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Halovenus anhuiensis sp. nov. and Natronomonas salinimetallica sp. nov., two extremely halophilic archaeon isolated from a salt mine

  • 期刊论文

  • 发表日期：

    2026年2月3日

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  • Ya-Ling Mao,
  • Aodi Zhang,
  • Tao Hong,
  • Yue Ding,
  • Jingfang Liu,
  • Jing Hou,
  • Heng-Lin Cui,
  • Shaoxing Chen

摘要

Two extremely halophilic archaeal strains, HT40T and LN261T, were isolated from salt crystal samples collected from the Dingyuan Salt Mine in Anhui, China. Strain HT40T was found to possess two copies of the 16 S rRNA gene (rrnA and rrnB), while only one copy was detected in strain LN261T. Phylogenetic analysis based on the 16 S rRNA gene sequences revealed that the rrnA and rrnB genes of HT40T showed high sequence similarities with Halovenus aranensis EB27T (94.73%) and Halovenus carboxidivorans WSH3T (97.42%), respectively. Strain LN261T was clustered with Natronomonas aquatica F2–12T, exhibiting 98.50% sequence similarity. Comparative genomic analysis demonstrated that the average nucleotide identity (ANI), digital DNA–DNA hybridization (dDDH), and average amino acid identity (AAI) values between HT40T and current species of the genus Halovenus ranged from 70.2 to 81.8%, 19.4–25.6%, and 62.2–83.8%, respectively. Similarly, the corresponding values between LN261T and current species of the genus Natronomonas were determined to be 75.0–87.3%, 21.2–38.8%, and 67.7–86.4%, respectively. All these values were significantly lower than the recommended thresholds for prokaryotic species delineation. Physiological and biochemical characterization indicated that the optimal growth conditions for HT40T were observed at 42 °C, 4.5 M NaCl, and pH 8.0, whereas LN261T exhibited optimal growth at 37 °C, 3.9 M NaCl, and pH 7.5. Based on the distinct phenotypic and genomic differences from the relevant species, strains HT40T (= MCCC 4K00167T = KCTC 4325T) and LN261T (= MCCC 4K00169T = CGMCC 1.17294T = KCTC 4321T) were proposed to represent novel species within the genera Halovenus and Natronomonas, respectively, for which the name Halovenus anhuiensis and Natronomonas salinimetallica are proposed.