Scholarlink

Introduction <p>Oral candidosis is very common and fungi resistance to drugs available on the market has been reported, as well as a high rate of recurrences. New treatment alternatives are studied.</p> Objective <p>This study developed a citral-based hydrogel (CBH), performed the physical-chemical characterization and evaluated, in vitro, the microbial susceptibility to <i>Candida</i> spp. and the growth kinetics. It also analyzed acute in vivo toxicity and mutagenicity through the micronucleus test in mouse erythrocytes.</p> Materials and methods <p>The following strains were used in the antifungal activity assays: <i>C. albicans</i> ATCC 10,231, <i>C. glabrata</i> ATCC 90,030, <i>C. krusei</i> ATCC 6258, <i>C. tropicalis</i> ATCC 750, <i>C. parapsilosis</i> ATCC 22,019 and clinical strains of <i>C. albicans</i> A4, <i>C. albicans</i> A5 and <i>C. albicans</i> A6. Microbial susceptibility was verified by the agar disk diffusion test and the effect on the growth kinetics of <i>C. albicans</i> (ATCC 10231). Acute toxicity and clastogenic and aneugenic potential were evaluated in <i>Mus Musculus</i> mice, line Swiss, age six weeks (CBH 2000&#xa0;mg/kg). Data were analyzed by descriptive and inferential ANOVA and Tukey statistics.</p> Results <p>CBH showed inhibition halos greater than or equal to 10&#xa0;mm in diameter depending on the concentration analyzed, for all <i>Candida</i> strains tested. The product significantly inhibited the growth of <i>C. albicans</i> ATCC 10,231 at all different inoculum concentrations evaluated, between 15 and 120&#xa0;min, when compared to the growth control. CBH did not induce signs of acute toxicity in mice submitted to gavage treatment at a single dose of 2000&#xa0;mg/kg. CBH produced a statistically significant smaller number of micronuclei compared to the positive control (cyclophosphomide 50&#xa0;mg/kg) in erythrocytes from mice.</p> Conclusion <p>The citral-based hydrogel proved to be a potential antifungal agent against <i>Candida</i> species, with low toxicity and mutagenicity.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Development of citral-based hydrogel for the treatment of oral candidosis: in vitro and in vivo study

  • 期刊论文

  • 发表日期：

    2025年10月20日

查看全文
  • Julliana Cariry Palhano Freire,
  • Adyelle Dantas Ribeiro,
  • Ernani Canuto Figueiredo Junior,
  • Dayanne Tomaz Casimiro da Silva,
  • Eduardo Dias Ribeiro,
  • Bolívar Ponciano Goulart de Lima Damasceno,
  • Pollianna Muniz Alves,
  • Cassiano Francisco Weege Nonaka,
  • Jozinete Vieira Pereira

摘要

Introduction

Oral candidosis is very common and fungi resistance to drugs available on the market has been reported, as well as a high rate of recurrences. New treatment alternatives are studied.

Objective

This study developed a citral-based hydrogel (CBH), performed the physical-chemical characterization and evaluated, in vitro, the microbial susceptibility to Candida spp. and the growth kinetics. It also analyzed acute in vivo toxicity and mutagenicity through the micronucleus test in mouse erythrocytes.

Materials and methods

The following strains were used in the antifungal activity assays: C. albicans ATCC 10,231, C. glabrata ATCC 90,030, C. krusei ATCC 6258, C. tropicalis ATCC 750, C. parapsilosis ATCC 22,019 and clinical strains of C. albicans A4, C. albicans A5 and C. albicans A6. Microbial susceptibility was verified by the agar disk diffusion test and the effect on the growth kinetics of C. albicans (ATCC 10231). Acute toxicity and clastogenic and aneugenic potential were evaluated in Mus Musculus mice, line Swiss, age six weeks (CBH 2000 mg/kg). Data were analyzed by descriptive and inferential ANOVA and Tukey statistics.

Results

CBH showed inhibition halos greater than or equal to 10 mm in diameter depending on the concentration analyzed, for all Candida strains tested. The product significantly inhibited the growth of C. albicans ATCC 10,231 at all different inoculum concentrations evaluated, between 15 and 120 min, when compared to the growth control. CBH did not induce signs of acute toxicity in mice submitted to gavage treatment at a single dose of 2000 mg/kg. CBH produced a statistically significant smaller number of micronuclei compared to the positive control (cyclophosphomide 50 mg/kg) in erythrocytes from mice.

Conclusion

The citral-based hydrogel proved to be a potential antifungal agent against Candida species, with low toxicity and mutagenicity.