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Objectives <p>The study aimed to develop and evaluate in vitro a varnish containing fluoride-doped glass ceramics capable of inhibiting oral microorganisms, releasing hydroxyapatite-forming ions, and ensuring biocompatibility.</p> Materials and methods <p>For the production of the experimental varnish (VE), composed of hydrogenated rosin, 10% and 20% by weight of glass ceramics with composition S53P4 (CF0) and doped with Fˉ ions (CF5 and CF10) were incorporated. The EVs were characterized by Rheology, Scanning Electron Microscopy (SEM), FTIR, ion release (fluoride and calcium ions), cytotoxicity on VERO cells, and antimicrobial effect on <i>S. mutans</i>, <i>S. aureus</i> and <i>C. albicans</i> and anti-biofilm effect on <i>S. mutans</i>. The data were analyzed by One-Way ANOVA and Kruskal–Wallis test (<i>P</i> = 0.05).</p> Results <p>Homogeneous varnishes with good viscosity were obtained. Varnishes with 20% CF demonstrated biocompatibility and minimum inhibitory concentration (MIC) at concentrations lower than 10&#xa0;mg/mL and 3.12&#xa0;mg/mL, respectively, except for <i>C. albicans</i>. An anti-biofilm effect on <i>S. mutans</i> was observed for the varnishes with 20% CF. All varnishes with CF released more Fˉ than the commercial varnish, with V20CF10 standing out, which released 4 times more Fˉ ions in a quarter of the time.</p> Conclusions <p>The V20CF10 varnish is a promising material for dental use in the treatment of early caries lesions, due to its biocompatibility, antimicrobial properties, and release of hydroxyapatite-forming ions.</p> <p>Clinical relevance.</p> <p>The use of a new varnish that combines antimicrobial properties with hydroxyapatite-forming ion release may prevent or halt the progression of dental caries lesions, offering greater efficacy than currently available varnishes.</p>

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Ion release, cytocompatibility and microbial inhibition of a novel varnish containing fluoride-doped bioactive glass ceramics: an in vitro study

  • 期刊论文

  • 发表日期：

    2025年2月16日

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  • Panmella Pereira Maciel,
  • Eudes Leonnan Gomes de Medeiros,
  • Lucas Ricardo Fernandes Figueiredo,
  • Rebeca Tibau Aguiar Dias,
  • Eduarda Gomes Onofre de Araújo,
  • Roberta Ferreti Bonan Dantas Batista,
  • Sara Brito Silva Costa Cruz,
  • Ricardo Dias de Castro,
  • Joelma Rodrigues de Souza,
  • Eliton Souto de Medeiros,
  • Romualdo Rodrigues Menezes,
  • André Ulisses Dantas Batista,
  • Paulo Rogério Ferreti Bonan

摘要

Objectives

The study aimed to develop and evaluate in vitro a varnish containing fluoride-doped glass ceramics capable of inhibiting oral microorganisms, releasing hydroxyapatite-forming ions, and ensuring biocompatibility.

Materials and methods

For the production of the experimental varnish (VE), composed of hydrogenated rosin, 10% and 20% by weight of glass ceramics with composition S53P4 (CF0) and doped with Fˉ ions (CF5 and CF10) were incorporated. The EVs were characterized by Rheology, Scanning Electron Microscopy (SEM), FTIR, ion release (fluoride and calcium ions), cytotoxicity on VERO cells, and antimicrobial effect on S. mutans, S. aureus and C. albicans and anti-biofilm effect on S. mutans. The data were analyzed by One-Way ANOVA and Kruskal–Wallis test (P = 0.05).

Results

Homogeneous varnishes with good viscosity were obtained. Varnishes with 20% CF demonstrated biocompatibility and minimum inhibitory concentration (MIC) at concentrations lower than 10 mg/mL and 3.12 mg/mL, respectively, except for C. albicans. An anti-biofilm effect on S. mutans was observed for the varnishes with 20% CF. All varnishes with CF released more Fˉ than the commercial varnish, with V20CF10 standing out, which released 4 times more Fˉ ions in a quarter of the time.

Conclusions

The V20CF10 varnish is a promising material for dental use in the treatment of early caries lesions, due to its biocompatibility, antimicrobial properties, and release of hydroxyapatite-forming ions.

Clinical relevance.

The use of a new varnish that combines antimicrobial properties with hydroxyapatite-forming ion release may prevent or halt the progression of dental caries lesions, offering greater efficacy than currently available varnishes.