Scholarlink

<p>In this study, a thin-layer chromatographic approach was developed for the enantioresolution of three chiral amine drugs, viz<i>.,</i> mexiletine, flecainide, and tocainide, using azithromycin (a macrocyclic antibiotic) as a chiral selector. Azithromycin was incorporated into the stationary phase on thin-layer chromatography (TLC) plates to create a stereochemically interactive microenvironment capable of promoting multiple molecular interactions, including hydrogen bonding, π–π stacking, dipole interactions, and steric recognition. The chromatographic conditions were optimized to achieve successful resolution of the racemic analytes. The limit of detection and limit of quantification values were found to be in the range of 0.072–0.108&#xa0;µg/spot and 0.216–0.321&#xa0;µg/spot, respectively, for the three analytes. This work demonstrates the successful application of azithromycin-modified TLC plates for the chiral resolution of the studied amine drugs, offering a simple, cost-efficient alternative for enantioseparation of chiral analytes and supporting further application in routine quality control and stereoselective pharmacokinetic studies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Chromatographic resolution of three chiral amines using chiral macrocyclic antibiotics as chiral selector

  • 期刊论文

  • 发表日期：

    2026年3月30日

查看全文
  • Gaurav Sharma,
  • Ramamohana Reddy Maddike,
  • Vivek Sharma,
  • Akash Kumar Mishra,
  • Vinod

摘要

In this study, a thin-layer chromatographic approach was developed for the enantioresolution of three chiral amine drugs, viz., mexiletine, flecainide, and tocainide, using azithromycin (a macrocyclic antibiotic) as a chiral selector. Azithromycin was incorporated into the stationary phase on thin-layer chromatography (TLC) plates to create a stereochemically interactive microenvironment capable of promoting multiple molecular interactions, including hydrogen bonding, π–π stacking, dipole interactions, and steric recognition. The chromatographic conditions were optimized to achieve successful resolution of the racemic analytes. The limit of detection and limit of quantification values were found to be in the range of 0.072–0.108 µg/spot and 0.216–0.321 µg/spot, respectively, for the three analytes. This work demonstrates the successful application of azithromycin-modified TLC plates for the chiral resolution of the studied amine drugs, offering a simple, cost-efficient alternative for enantioseparation of chiral analytes and supporting further application in routine quality control and stereoselective pharmacokinetic studies.