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<p>The increased demand for medicinal plants such as <i>Rheum emodi</i> Wall. Ex Meisn. has intensified exploitation owing to its therapeutic properties. The plant is in high demand, often leading to adulteration or substitution with related species. This study aimed to develop a validated thin-layer chromatography (TLC) method for identification of key variables for precise species authentication and adulteration detection. Samples were initially identified using macroscopic and microscopic analysis, but high microscopic similarity highlights the need for standardization using advanced analytical technique. The ethanolic extracts of the test and standard samples were evaluated using the TLC technique, which was tested for linearity, specificity, precision, and accuracy, in compliance with the International Council for Harmonisation (ICH) standards. The quantitative assessment was carried out using pure emodin as external standard. By comparing the test samples with the reference standard, the chemicals’ identities were verified, which could help in distinguishing the original drug from its adulterants. The mobile phase hexane–ethyl acetate–formic acid (7:2.5:0.5, <i>V/V</i>) helped in the analysis of emodin in <i>R. emodi</i> and its adulterants at 254&#xa0;nm. The technical data generated clearly showed that the genuine <i>R. emodi</i> sample contained the highest amount of the principal compound emodin, thus guaranteeing the genuineness and quality of <i>R. emodi</i>.</p>

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Comparative study of different Rheum species to establish qualitative and quantitative standards for identification of Rheum emodi’s adulterants

  • 期刊论文

  • 发表日期：

    2026年3月18日

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  • Anupam Kumar Mangal,
  • Salik Abdullah,
  • Jyoti Dahiya,
  • Rajesh Bolleddu,
  • Ijaz Ahmed,
  • Ankita Ghosh,
  • Nagayya Shiddamallayya,
  • Govindarajan Nartunai,
  • Vendrapati Rama Rao,
  • Animesh Sen,
  • Padma Gurmet,
  • Gajji Babu,
  • Narayanam Srikanth,
  • Rabinarayan Acharya

摘要

The increased demand for medicinal plants such as Rheum emodi Wall. Ex Meisn. has intensified exploitation owing to its therapeutic properties. The plant is in high demand, often leading to adulteration or substitution with related species. This study aimed to develop a validated thin-layer chromatography (TLC) method for identification of key variables for precise species authentication and adulteration detection. Samples were initially identified using macroscopic and microscopic analysis, but high microscopic similarity highlights the need for standardization using advanced analytical technique. The ethanolic extracts of the test and standard samples were evaluated using the TLC technique, which was tested for linearity, specificity, precision, and accuracy, in compliance with the International Council for Harmonisation (ICH) standards. The quantitative assessment was carried out using pure emodin as external standard. By comparing the test samples with the reference standard, the chemicals’ identities were verified, which could help in distinguishing the original drug from its adulterants. The mobile phase hexane–ethyl acetate–formic acid (7:2.5:0.5, V/V) helped in the analysis of emodin in R. emodi and its adulterants at 254 nm. The technical data generated clearly showed that the genuine R. emodi sample contained the highest amount of the principal compound emodin, thus guaranteeing the genuineness and quality of R. emodi.