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<p><i>Euphorbia hirta</i> L., belonging to the Euphorbiaceae family, possesses a rich ethnopharmacological background and displays a broad range of biological properties. Despite evidence of the efficacy of the crude extract against <i>Candida</i> sp., the bioactive compounds underlying the anti-candidal properties have not been isolated or fully characterized. In this study, we investigated the anti-candidal potential of <i>E. hirta</i> and observed significant activity against multidrug-resistant and susceptible <i>Candida albicans</i>. Bioassay-guided purification using high-performance thin-layer chromatography (HPTLC) successfully led to the isolation and purification of the targeted anti-candidal compound. The isolated compound kaempferol, which demonstrated significant anti-candidal activity against multidrug-resistant and susceptible <i>C. albicans</i> strains, with MIC values of 0.25&#xa0;μg/mL and 0.125&#xa0;μg/mL, respectively. Kaempferol also exhibited a favorable cytotoxicity profile, with a cytotoxic concentration 50% (CC<sub>50</sub>) value of 124.64&#xa0;μg/mL in the VERO cell line. This study highlights the efficacy of HPTLC in the rapid identification and isolation of bioactive compounds from complex plant extracts, demonstrating the potential for streamlining the discovery and development of novel natural products with therapeutic potential.</p>

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Anti-candidal directed isolation of kaempferol from Euphorbia hirta against multidrug-resistant Candida albicans: a high-performance thin-layer chromatography-based approach

  • 期刊论文

  • 发表日期：

    2025年6月1日

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  • Smija Kakkuth Puthenvitil,
  • Palani Perumal

摘要

Euphorbia hirta L., belonging to the Euphorbiaceae family, possesses a rich ethnopharmacological background and displays a broad range of biological properties. Despite evidence of the efficacy of the crude extract against Candida sp., the bioactive compounds underlying the anti-candidal properties have not been isolated or fully characterized. In this study, we investigated the anti-candidal potential of E. hirta and observed significant activity against multidrug-resistant and susceptible Candida albicans. Bioassay-guided purification using high-performance thin-layer chromatography (HPTLC) successfully led to the isolation and purification of the targeted anti-candidal compound. The isolated compound kaempferol, which demonstrated significant anti-candidal activity against multidrug-resistant and susceptible C. albicans strains, with MIC values of 0.25 μg/mL and 0.125 μg/mL, respectively. Kaempferol also exhibited a favorable cytotoxicity profile, with a cytotoxic concentration 50% (CC50) value of 124.64 μg/mL in the VERO cell line. This study highlights the efficacy of HPTLC in the rapid identification and isolation of bioactive compounds from complex plant extracts, demonstrating the potential for streamlining the discovery and development of novel natural products with therapeutic potential.