Purpose <p>Antenatal depression is a common psychological condition in pregnancy that negatively influences parenting. Theoretical models suggest that infant cue processing may represent one pathway by which depression may influence parenting. However, current understanding of how infant cue processing is linked with parenting in depression remains limited. Drawing upon previous research, cortisol stress reactivity may play an important role in this regard. The aim of the present study was to investigate the interaction between depressive symptoms and attentional disengagement from infant cues on cortisol reactivity. We examined this in a sample of pregnant and nulliparous women to test whether potential effects were specific to pregnancy.</p> Methods <p><i>N</i> = 79 women (<i>n</i> = 36 pregnant) completed two eye-tracking tasks examining disengagement from adult and infant stimuli, a stress manipulation task including collection of salivary cortisol, and filled out the BDI-II.</p> Results <p>Pregnant (vs. nulliparous) women showed a stronger cortisol reactivity in response to a stress test. Additionally, a significant association between disengagement from distressed infant faces and cortisol reactivity was found in the pregnant (but not nulliparous) group, which was moderated by depressive symptoms. For pregnant women with low levels of depressive symptoms, a slower disengagement from distressed infant stimuli predicted a weaker cortisol reactivity in response to stress, while the opposite pattern was observed for pregnant women with high levels of depressive symptoms.</p> Conclusions <p>Findings of the present study point at maternal distress during processing of infant distress as a potential intervention target for mothers with depression.</p>