Scholarlink

<p>It is generally accepted that amino acids (AAs) digestibility should be evaluated at the ileum level. A study was conducted, to assess the effects of varying levels of rumen-protected methionine (RPMet) supplementation, with low crude protein (CP) diet, on the total AA flow from rumen to the small intestine (ileum) using the rumen simulation technique (Rusitec) along with in vitro abomasum and ileum digestibility methods. The randomized, independent study was carried out twice over 15-day incubation periods. Four diets were tested: (a) a high-protein diet (163.39&#xa0;g/kg; HP), (b) a low-protein diet (146.33&#xa0;g/kg; LP), (c) a low-protein diet supplemented with low RPMet (0.11&#xa0;g/kg; LPLMet), and (d) a low-protein diet supplemented with high RPMet (0.81&#xa0;g/kg; LPHMet). Each diet was mixed with 20 ± 0.04&#xa0;g of a basal diet in each fermenter of Rusitec system. The results showed that methionine (Met) flow from the Rusitec and abomasum residue was not significantly affected (<i>P</i> &gt; 0.05) across all groups. However, total AA flow was significantly higher (<i>P</i> &lt; 0.05) in the HP group than in the LP, LPLMet, and LPHMet groups in both the Rusitec and in vitro abomasum studies. The supplementation of RPMet with low CP diets significantly (<i>P</i> &lt; 0.05) influenced the total and Met AA flow from the Rusitec to the ileum in the fluid portion. However, total AA flow was significantly higher (<i>P</i> &lt; 0.05) in the HP group compared to other groups in the Rusitec fluid. Although at the abomasum and ileum sites, it was comparable (<i>P</i> &gt; 0.05) between HP &amp; LPHMet and higher than in the other groups. Furthermore, methionine flow remained unaffected among groups (<i>P</i> &gt; 0.05) in the Rusitec fermenter but was significantly higher (<i>P</i> &lt; 0.05) in the LPHMet at the abomasum and ileum than in the other groups. In conclusion,high level of rumen-protected methionine (RPMet) supplementation with low-protein diet, can increase amino acid supply in intestine at the same rate as higher-protein diet (HP). This helps to ensure that low crude protein diet with RPMet, provide adequate delivery of amino acids, and reduce feed expense with nitrogen losses in environment. </p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Estimate ileal flow of methionine and total amino acids; supplemented rumen-protected methionine with low dietary protein diet using different in vitro digestibility techniques

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年8月5日

查看全文
  • Imtiaz Hussain Raja,
  • Farzana Abbasi,
  • Yangchun Cao

摘要

It is generally accepted that amino acids (AAs) digestibility should be evaluated at the ileum level. A study was conducted, to assess the effects of varying levels of rumen-protected methionine (RPMet) supplementation, with low crude protein (CP) diet, on the total AA flow from rumen to the small intestine (ileum) using the rumen simulation technique (Rusitec) along with in vitro abomasum and ileum digestibility methods. The randomized, independent study was carried out twice over 15-day incubation periods. Four diets were tested: (a) a high-protein diet (163.39 g/kg; HP), (b) a low-protein diet (146.33 g/kg; LP), (c) a low-protein diet supplemented with low RPMet (0.11 g/kg; LPLMet), and (d) a low-protein diet supplemented with high RPMet (0.81 g/kg; LPHMet). Each diet was mixed with 20 ± 0.04 g of a basal diet in each fermenter of Rusitec system. The results showed that methionine (Met) flow from the Rusitec and abomasum residue was not significantly affected (P > 0.05) across all groups. However, total AA flow was significantly higher (P < 0.05) in the HP group than in the LP, LPLMet, and LPHMet groups in both the Rusitec and in vitro abomasum studies. The supplementation of RPMet with low CP diets significantly (P < 0.05) influenced the total and Met AA flow from the Rusitec to the ileum in the fluid portion. However, total AA flow was significantly higher (P < 0.05) in the HP group compared to other groups in the Rusitec fluid. Although at the abomasum and ileum sites, it was comparable (P > 0.05) between HP & LPHMet and higher than in the other groups. Furthermore, methionine flow remained unaffected among groups (P > 0.05) in the Rusitec fermenter but was significantly higher (P < 0.05) in the LPHMet at the abomasum and ileum than in the other groups. In conclusion,high level of rumen-protected methionine (RPMet) supplementation with low-protein diet, can increase amino acid supply in intestine at the same rate as higher-protein diet (HP). This helps to ensure that low crude protein diet with RPMet, provide adequate delivery of amino acids, and reduce feed expense with nitrogen losses in environment.