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<p>This study aimed to elucidate hepatic metabolic alterations in diet-induced fatty liver disease using a combination of high-field in vivo magnetic resonance spectroscopy (MRS) and ex vivo high-resolution NMR-based metabolomics. Specifically, we focused on the correlations between lipid accumulation and metabolic profiles. Livers from high-fat diet (HFD) fed mice (<i>n</i> = 19) and standard diet controls (<i>n</i> = 14) were examined. In vivo MRS was performed to analyze the composition of lipid protons (LP). Subsequently, liver tissue extracts were analyzed using 700&#xa0;MHz NMR spectroscopy for comprehensive metabolomic profiling. Multivariate statistical analyses were employed to identify significant metabolic differences and their associations with lipid content. The HFD group exhibited a significant increase in intrahepatic fat, characterized by distinct LP ratios at 1.30, 2.25, 2.78, and 5.30&#xa0;ppm. Metabolomic analysis revealed significantly decreased levels of six key metabolites in the HFD group: alanine, betaine, creatine, formate, glycine, and succinate. Correlation analyses established a strong relationship between specific LP signals and these metabolites, indicating that alterations in amino acid and energy metabolism are closely associated with the development of hepatic steatosis. Our findings highlight a distinct metabolic signature for Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD). The combined approach of in vivo MRS and high-resolution NMR serves as a powerful tool for identifying potential non-invasive biomarkers and advancing the understanding of the pathophysiological mechanisms of fatty liver disease.</p>

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Integrated In vivo MRS and Ex vivo NMR Metabolomics Reveal Metabolic Signatures of Diet-Induced Fatty Liver in Mice

  • 期刊论文

  • 发表日期：

    2026年5月25日

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  • Chea-Young Lee,
  • Seo-Young Kim,
  • Sooah Kim,
  • Seung-Man Yu

摘要

This study aimed to elucidate hepatic metabolic alterations in diet-induced fatty liver disease using a combination of high-field in vivo magnetic resonance spectroscopy (MRS) and ex vivo high-resolution NMR-based metabolomics. Specifically, we focused on the correlations between lipid accumulation and metabolic profiles. Livers from high-fat diet (HFD) fed mice (n = 19) and standard diet controls (n = 14) were examined. In vivo MRS was performed to analyze the composition of lipid protons (LP). Subsequently, liver tissue extracts were analyzed using 700 MHz NMR spectroscopy for comprehensive metabolomic profiling. Multivariate statistical analyses were employed to identify significant metabolic differences and their associations with lipid content. The HFD group exhibited a significant increase in intrahepatic fat, characterized by distinct LP ratios at 1.30, 2.25, 2.78, and 5.30 ppm. Metabolomic analysis revealed significantly decreased levels of six key metabolites in the HFD group: alanine, betaine, creatine, formate, glycine, and succinate. Correlation analyses established a strong relationship between specific LP signals and these metabolites, indicating that alterations in amino acid and energy metabolism are closely associated with the development of hepatic steatosis. Our findings highlight a distinct metabolic signature for Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD). The combined approach of in vivo MRS and high-resolution NMR serves as a powerful tool for identifying potential non-invasive biomarkers and advancing the understanding of the pathophysiological mechanisms of fatty liver disease.