<p>Adult males of <i>Podisus nigrispinus</i> Dallas (Heteroptera: Pentatomidae) produce pheromones in their dorsal-abdominal glands, which are located internally between abdominal tergites III and IV. These pheromones attract adult individuals making them useful for biological control and as bait for capture. This article presents a morphological description of the dorsal-abdominal glands in male <i>P. nigrispinus</i> using light microscopy, as well as scanning and transmission electron microscopy. The glands consist of a pair of coiled tubular secretory structures, each opening in an enlarged reservoir. The glandular epithelium is composed of three cell layers: a basal layer of flattened cells, a middle layer of both columnar secretory cells and narrowed interstitial cells, and an apical layer of duct cells. The duct cells enclose a glandular reservoir lined with a cuticle, into which the secretory cells release their products through a sinuous intracellular duct. Each glandular reservoir opens outward through a pair of ostioles in the dorsal tergite, controlled by a tegumental valve. Cytoplasmic characteristics suggest that the secretory product is likely synthesized through fatty acid metabolism, similar to the sex pheromones produced by other insects.</p>