Exploring cadmium uptake pathways in the roots of Coptis chinensis Franch

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发表日期：

2025年3月5日

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Yuting Niu,
Shuang He,
Lu Xing,
Dongdong Zhang,
Yuze Li,
Chong Deng,
Yi Jiang,
Huawei Zhang,
Xiaomei Song,
Meihai Ding,
Wenli Huang,
Wei Wang

摘要

To investigate the cadmium (Cd) uptake mechanism in the roots of Coptis chinensis Franch., we utilized non-invasive micro-test technology (NMT) to analyze Cd²⁺ and H⁺ fluxes, alongside ICP-MS to assess Cd content and fluxes under various inhibitors and ion channel blockers. Results revealed that Cd²⁺ uptake primarily occurs in the root meristematic zone. Cd²⁺ addition significantly inhibited H⁺ influx. The P-type ATPase inhibitor Na₃VO₄ and the metabolism inhibitor CCCP both reduced Cd²⁺ fluxes and Cd content in roots of C. chinensis Ca²⁺ channel blocker LaCl₃ exhibited more pronounced inhibition on Cd²⁺ flux than K⁺ channel blocker TEA, indicating Cd²⁺ uptake mainly via Ca²⁺ channels. Therefore, active transport and Ca²⁺ channels may play an important role in Cd uptake in C. chinensis roots.