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<p>This work deals with protoporphyrin IX (PPIX) response to continual UVA and UVB irradiation and white light treatments in four different mediums (two aqueous, one chloroform, and one lipid mixture) by using ultra-high-performance liquid chromatography coupled with diode array and mass spectrometry detection (UHPLC-DAD-ESI–MS/MS), providing consideration of possible photoproducts formation as well as photodegradation kinetics. As a result of the treatments, several photooxidation products such as photoprotoporphyrins and formylporphyrins were detected in the mediums with some new and not fully identified products in chloroform, most probable originating from further photooxidation of the PPIX. Interaction of PPIX with UV radiation and white light induces its photodegradation in selected mediums, obeying first-order kinetics. The results shown in this work suggest complex kinetics dependence on different factors such as PPIX molecular organization, photosensitized singlet oxygen production, the nature of solvent and solvent-dependent singlet oxygen lifetime in the mediums, as well as absorption ability of PPIX in used emission spectral ranges of the treatments.</p> Graphical abstract <p></p>

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Protoporphyrin IX response to ultraviolet and white light treatments in different mediums

  • 期刊论文

  • 发表日期：

    2025年3月22日

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  • Aleksandar Lazarević,
  • Sanja Petrović,
  • Dragan Cvetković,
  • Jelena Stanojević,
  • Tatjana Anđelković,
  • Jelena Zvezdanović

摘要

This work deals with protoporphyrin IX (PPIX) response to continual UVA and UVB irradiation and white light treatments in four different mediums (two aqueous, one chloroform, and one lipid mixture) by using ultra-high-performance liquid chromatography coupled with diode array and mass spectrometry detection (UHPLC-DAD-ESI–MS/MS), providing consideration of possible photoproducts formation as well as photodegradation kinetics. As a result of the treatments, several photooxidation products such as photoprotoporphyrins and formylporphyrins were detected in the mediums with some new and not fully identified products in chloroform, most probable originating from further photooxidation of the PPIX. Interaction of PPIX with UV radiation and white light induces its photodegradation in selected mediums, obeying first-order kinetics. The results shown in this work suggest complex kinetics dependence on different factors such as PPIX molecular organization, photosensitized singlet oxygen production, the nature of solvent and solvent-dependent singlet oxygen lifetime in the mediums, as well as absorption ability of PPIX in used emission spectral ranges of the treatments.

Graphical abstract