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<p>A novel piscine adenovirus, <i>Pagrus major</i> adenovirus 1 (PmAdV-1), was identified in red seabream (<i>Pagrus major</i>) by metagenomic sequencing. The 29,519 bp genome encodes 22 predicted open reading frames and exhibits a unique organization, with the fiber gene positioned upstream of the conserved adenovirus gene cluster. Phylogenetic analyses indicate that PmAdV-1 forms a sister lineage to red-eared slider adenovirus 1 within a clade of fish and reptilian adenoviruses, but its assignment to the genus <i>Testadenovirus</i> remains uncertain. A virus-specific qPCR assay was developed to monitor PmAdV-1 in environmental DNA from rearing seawater. Viral loads transiently increased in some juvenile tanks without marked mortality. These findings expand current knowledge of fish adenovirus diversity.</p>

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Genome characterization and environmental DNA-based detection of a novel adenovirus from red seabream (Pagrus major)

  • 期刊论文

  • 发表日期：

    2026年4月15日

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  • Naritoyo Ishibashi,
  • Yuri Akase,
  • Atsuko Ito,
  • Kenta Kishimoto,
  • Shuntaro Watanabe,
  • Hiroshi Yokoyama,
  • Tohru Mekata

摘要

A novel piscine adenovirus, Pagrus major adenovirus 1 (PmAdV-1), was identified in red seabream (Pagrus major) by metagenomic sequencing. The 29,519 bp genome encodes 22 predicted open reading frames and exhibits a unique organization, with the fiber gene positioned upstream of the conserved adenovirus gene cluster. Phylogenetic analyses indicate that PmAdV-1 forms a sister lineage to red-eared slider adenovirus 1 within a clade of fish and reptilian adenoviruses, but its assignment to the genus Testadenovirus remains uncertain. A virus-specific qPCR assay was developed to monitor PmAdV-1 in environmental DNA from rearing seawater. Viral loads transiently increased in some juvenile tanks without marked mortality. These findings expand current knowledge of fish adenovirus diversity.