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<p>A putative novel tri-segmented rhabdovirus, tentatively named Humulus trirhavirus 1 (HuTRV1), was identified from mosaic and chlorotic leaves of <i>Humulus scandens</i> in Shenyang, Liaoning, China. Using high-throughput sequencing, RT-PCR and RACE, HuTRV1 was found to comprise three genomic segments (RNA1, RNA2, RNA3) of 6,674, 4,417, and 4,094 nucleotides (nt) respectively. Its genome organization resembles that of Chenopodium trirhavirus 1 identified from <i>Chenopodium album</i> in China, containing eight open reading frames (ORFs) encoding putative L, N, P2-P4, and P6-P8 proteins. All these proteins shared less than 58.6% nt and 51.9% amino acid (aa) sequence identity with other known rhabdoviruses. Phylogenetic analysis of the L protein sequences confirmed the close relationship of HuTRV1 with trirhaviruses in a distinct clade in the family <i>Rhabdoviridae</i>. These results suggest that HuTRV1 represents a new <i>Trirhavirus</i> species, for which we propose the name <i>Trirhavirus humuli</i>.</p>

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Identification and characterization of a putative novel tri-segmented rhabdovirus infecting Humulus scandens

  • 期刊论文

  • 发表日期：

    2026年4月7日

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  • Jing Wang,
  • Yujie Li,
  • Yanyi Zhang,
  • Song Zhang,
  • Mengji Cao,
  • Caixia Yang

摘要

A putative novel tri-segmented rhabdovirus, tentatively named Humulus trirhavirus 1 (HuTRV1), was identified from mosaic and chlorotic leaves of Humulus scandens in Shenyang, Liaoning, China. Using high-throughput sequencing, RT-PCR and RACE, HuTRV1 was found to comprise three genomic segments (RNA1, RNA2, RNA3) of 6,674, 4,417, and 4,094 nucleotides (nt) respectively. Its genome organization resembles that of Chenopodium trirhavirus 1 identified from Chenopodium album in China, containing eight open reading frames (ORFs) encoding putative L, N, P2-P4, and P6-P8 proteins. All these proteins shared less than 58.6% nt and 51.9% amino acid (aa) sequence identity with other known rhabdoviruses. Phylogenetic analysis of the L protein sequences confirmed the close relationship of HuTRV1 with trirhaviruses in a distinct clade in the family Rhabdoviridae. These results suggest that HuTRV1 represents a new Trirhavirus species, for which we propose the name Trirhavirus humuli.