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<p>A convenient tool for identifying glycan receptors of new influenza virus strains is the PGA (printed glycan array), which contains up to a hundred sialoglycans. However, antigen diversity and drift complicate the detection of the virus bound to PGA by antibodies, since, generally speaking, new strain is needed for a new antibody. In this work, the bound virus was visualized on PGA using labeled sialopolymers, namely Neu5Acα2-3Galβ1-4GlcNAcβ-PAA-biotin (for avian viruses) or Neu5Acα2-6Galβ1-4GlcNAcβ-PAA-biotin (for human viruses), where PAA is soluble polyacrylamide. Detection of strains H6N1(Sarma/51c/2006), H7N1(FPV/Rostock/2007) and B19 (Moscow/19/2013) was routinely performed at a level of 16 hemagglutination units.</p>

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Antibody-free detection of influenza viruses on a microarray to study their receptor specificity

  • 期刊论文

  • 发表日期：

    2026年2月19日

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  • N. V. Shilova,
  • D. R. Yermolaeva,
  • A. Yu Nokel,
  • A. A. Chinarev,
  • G. V. Pazynina,
  • M. A. Sablina,
  • E. A. Gordeeva,
  • A. B. Komissarov,
  • N. V. Bovin

摘要

A convenient tool for identifying glycan receptors of new influenza virus strains is the PGA (printed glycan array), which contains up to a hundred sialoglycans. However, antigen diversity and drift complicate the detection of the virus bound to PGA by antibodies, since, generally speaking, new strain is needed for a new antibody. In this work, the bound virus was visualized on PGA using labeled sialopolymers, namely Neu5Acα2-3Galβ1-4GlcNAcβ-PAA-biotin (for avian viruses) or Neu5Acα2-6Galβ1-4GlcNAcβ-PAA-biotin (for human viruses), where PAA is soluble polyacrylamide. Detection of strains H6N1(Sarma/51c/2006), H7N1(FPV/Rostock/2007) and B19 (Moscow/19/2013) was routinely performed at a level of 16 hemagglutination units.