Scholarlink

<p><i>Klebsiella pneumoniae</i> (<i>K. pneumoniae</i>) is a leading cause of both nosocomial and community-acquired infections. In this study, we isolated a lytic bacteriophage (phage), designated Kpp-61, targeting <i>K. pneumoniae</i> with capsular serotype K1, which is prevalent in hypervirulent strains. Transmission electron microscopy showed that Kpp-61 had an icosahedral head and a non-contractile short tail. In vitro, Kpp-61 demonstrated significant antibiofilm activity, inhibiting biofilm formation and disrupting preformed biofilms. Furthermore, in a murine infection model, Kpp-61 improved the 7-day survival rate of mice to 60% and 90% at MOIs of 10 and 100, respectively, and mitigated pneumonia symptoms in <i>K. pneumoniae</i> strain Kp61-infected mice. Notably, Kpp-61 exhibited high specificity for K1-serotype strains, along with robust thermal and pH stability. Genomic analysis revealed a circular double-stranded DNA genome of 44,028&#xa0;bp, with a G + C content of 50.28% and 54 predicted open reading frames. Crucially, no virulence or antibiotic-resistance genes were detected. These results indicate the therapeutic potential of phage Kpp-61 against K1-serotype <i>K. pneumoniae</i> infections.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Biological characterization and genomic analysis of Kpp-61, a lytic bacteriophage against K1-type Klebsiella pneumoniae

  • 期刊论文

  • 发表日期：

    2026年2月16日

查看全文
  • Xin Jiao,
  • Shuqi Yang,
  • Jiayi Liu,
  • Shuo Xu,
  • Jiahui Wu,
  • Xiao Dong,
  • Jinjuan Qiao

摘要

Klebsiella pneumoniae (K. pneumoniae) is a leading cause of both nosocomial and community-acquired infections. In this study, we isolated a lytic bacteriophage (phage), designated Kpp-61, targeting K. pneumoniae with capsular serotype K1, which is prevalent in hypervirulent strains. Transmission electron microscopy showed that Kpp-61 had an icosahedral head and a non-contractile short tail. In vitro, Kpp-61 demonstrated significant antibiofilm activity, inhibiting biofilm formation and disrupting preformed biofilms. Furthermore, in a murine infection model, Kpp-61 improved the 7-day survival rate of mice to 60% and 90% at MOIs of 10 and 100, respectively, and mitigated pneumonia symptoms in K. pneumoniae strain Kp61-infected mice. Notably, Kpp-61 exhibited high specificity for K1-serotype strains, along with robust thermal and pH stability. Genomic analysis revealed a circular double-stranded DNA genome of 44,028 bp, with a G + C content of 50.28% and 54 predicted open reading frames. Crucially, no virulence or antibiotic-resistance genes were detected. These results indicate the therapeutic potential of phage Kpp-61 against K1-serotype K. pneumoniae infections.