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<p>Kiwifruit (<i>Actinidia</i> spp.) is a widely popular fruit crop worldwide with significant economic value. From kiwifruit leaves exhibiting symptoms of chlorosis and mosaic in Shaanxi Province of China, a novel tymovirus tentatively named “Actinidia tymovirus 1” (AcTmV1) was identified by high-throughput sequencing, and its complete genome sequence was determined by RT-PCR and RACE technologies. The genome of AcTmV1 with a length of 6,155 nucleotides (nt) contains three open reading frames (ORFs) encoding movement protein (MP) of 610 amino acids (aa), replicase polyprotein (RP) of 1,782 aa and coat protein (CP) of 187 aa, exhibiting typical tymovirus-like organization. The AcTmV1 genome shares the highest nt sequence identity of 64.5% to that of Valeriana jatamansi tymovirus 1 (VaJV1) (GenBank No. OQ730267), and CP shares the highest aa sequence identity of 55.1% to that of Kennedya yellow mosaic virus (KYMV) (GenBank No. D00637). Phylogenetic analyses based on both genome nt and CP aa sequences confirmed that AcTmV1 is most closely related to VaJV1. Therefore, it is proposed that AcTmV1 represents a new member of the genus <i>Tymovirus</i>.</p>

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Genomic characterization of actinidia tymovirus 1 (AcTmV1), a novel member of the genus Tymovirus infecting Kiwifruit

  • 期刊论文

  • 发表日期：

    2026年2月19日

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  • Huan Liu,
  • Wenhui Zhang,
  • Xiaodong Tang,
  • Wei Wu,
  • Shuang Song

摘要

Kiwifruit (Actinidia spp.) is a widely popular fruit crop worldwide with significant economic value. From kiwifruit leaves exhibiting symptoms of chlorosis and mosaic in Shaanxi Province of China, a novel tymovirus tentatively named “Actinidia tymovirus 1” (AcTmV1) was identified by high-throughput sequencing, and its complete genome sequence was determined by RT-PCR and RACE technologies. The genome of AcTmV1 with a length of 6,155 nucleotides (nt) contains three open reading frames (ORFs) encoding movement protein (MP) of 610 amino acids (aa), replicase polyprotein (RP) of 1,782 aa and coat protein (CP) of 187 aa, exhibiting typical tymovirus-like organization. The AcTmV1 genome shares the highest nt sequence identity of 64.5% to that of Valeriana jatamansi tymovirus 1 (VaJV1) (GenBank No. OQ730267), and CP shares the highest aa sequence identity of 55.1% to that of Kennedya yellow mosaic virus (KYMV) (GenBank No. D00637). Phylogenetic analyses based on both genome nt and CP aa sequences confirmed that AcTmV1 is most closely related to VaJV1. Therefore, it is proposed that AcTmV1 represents a new member of the genus Tymovirus.