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<p>Here, we describe a novel ourmia-like virus, Botryosphaeria dothidea ourmia-like virus 3 (BdOLV3), obtained from the phytopathogenic fungus <i>Botryosphaeria dothidea</i> strain ZM211299-2 infecting apple shoots in Shandong province of China. The genome of BdOLV3 is a positive-sense single-stranded RNA with a length of 2,650 nucleotides (nt), containing a large open reading frame (ORF) that encodes a putative RNA-dependent RNA polymerase (RdRp) consisting of 673 amino acids (aa) with a molecular mass of 75.53 kDa. This RdRp protein contains eight typical conserved motifs associated with ourmia-like viruses. BLASTp analysis revealed that the RdRp protein of BdOLV3 had the highest similarity (61.98%, 61.83%, and 51.47% identity, respectively) to viruses previously identified as Plasmopara viticola lesion associated ourmia-like virus 45, Erysiphe necator associated ourmia-like virus 130, and Magnaporthe oryzae botourmiavirus 7. Phylogenetic analysis based on RdRp sequences indicated that BdOLV3 is a new member of the genus <i>Epsilonscleroulivirus</i> in the family <i>Botourmiaviridae</i>. This is the first report of the complete genomic sequence of a member of the genus <i>Epsilonscleroulivirus</i> found in <i>B. dothidea</i>.</p>

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Molecular characterization of the (+)ssRNA genome of an ourmia-like virus infecting the plant-pathogenic fungus Botryosphaeria dothidea

  • 期刊论文

  • 发表日期：

    2026年1月7日

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  • Shunpei Xie,
  • Haiyan Wu,
  • Fengyue Gong,
  • Xiuyuan Sun,
  • Ruobing Kong,
  • Qinzhou Ma,
  • Yashuang Guo,
  • Haiqiang Li,
  • Lirong Yang,
  • Chao Xu,
  • Meng Zhang

摘要

Here, we describe a novel ourmia-like virus, Botryosphaeria dothidea ourmia-like virus 3 (BdOLV3), obtained from the phytopathogenic fungus Botryosphaeria dothidea strain ZM211299-2 infecting apple shoots in Shandong province of China. The genome of BdOLV3 is a positive-sense single-stranded RNA with a length of 2,650 nucleotides (nt), containing a large open reading frame (ORF) that encodes a putative RNA-dependent RNA polymerase (RdRp) consisting of 673 amino acids (aa) with a molecular mass of 75.53 kDa. This RdRp protein contains eight typical conserved motifs associated with ourmia-like viruses. BLASTp analysis revealed that the RdRp protein of BdOLV3 had the highest similarity (61.98%, 61.83%, and 51.47% identity, respectively) to viruses previously identified as Plasmopara viticola lesion associated ourmia-like virus 45, Erysiphe necator associated ourmia-like virus 130, and Magnaporthe oryzae botourmiavirus 7. Phylogenetic analysis based on RdRp sequences indicated that BdOLV3 is a new member of the genus Epsilonscleroulivirus in the family Botourmiaviridae. This is the first report of the complete genomic sequence of a member of the genus Epsilonscleroulivirus found in B. dothidea.