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<p>In this study, we identified and characterized two mycoviruses from the <i>Nigrospora oryzae</i> strain WK-26: <Emphasis Type="Underline">N</Emphasis>igrospora <Emphasis Type="Underline">o</Emphasis>ryzae <Emphasis Type="Underline">m</Emphasis>ito<Emphasis Type="Underline">v</Emphasis>irus <Emphasis Type="Underline">4</Emphasis> (NoMV4) and <Emphasis Type="Underline">N</Emphasis>igrospora <Emphasis Type="Underline">o</Emphasis>ryzae bot<Emphasis Type="Underline">o</Emphasis>u<Emphasis Type="Underline">l</Emphasis>i<Emphasis Type="Underline">v</Emphasis>irus <Emphasis Type="Underline">1</Emphasis> (NoOLV1). The genome of NoMV4 is 2529 nucleotides in length, containing a single open reading frame (ORF) that encodes an RNA-dependent RNA polymerase (RdRp). BLASTp analysis revealed that NoMV4 shares the highest sequence similarity (53.06% identity) with Fusarium graminearum mitovirus 1. Phylogenetic analysis indicated that NoMV4 belongs to the genus <i>Unuamitovirus</i> within the family <i>Mitoviridae</i>. The genome of NoOLV1 is 2951 nucleotides in length and encodes an RdRp protein with the highest sequence similarity (64.46% identity) to that of Nigrospora sphaerica ourmia-like virus 1. Phylogenetic analysis suggested that NoOLV1 is a new member of the genus <i>Deltascleroulivirus</i> within the family <i>Botourmiaviridae</i>. This is the first report of the complete genome sequence of a botoulivirus in <i>N. oryzae</i>.</p>

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Molecular characterization of a novel mitovirus and a botoulivirus coinfecting the fungus Nigrospora oryzae

  • 期刊论文

  • 发表日期：

    2025年11月3日

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  • Jinyue Zou,
  • Lihong Cheng,
  • Yitong Chen,
  • Baojun Zhang,
  • Fan Mu,
  • Jichun Jia

摘要

In this study, we identified and characterized two mycoviruses from the Nigrospora oryzae strain WK-26: Nigrospora oryzae mitovirus 4 (NoMV4) and Nigrospora oryzae botoulivirus 1 (NoOLV1). The genome of NoMV4 is 2529 nucleotides in length, containing a single open reading frame (ORF) that encodes an RNA-dependent RNA polymerase (RdRp). BLASTp analysis revealed that NoMV4 shares the highest sequence similarity (53.06% identity) with Fusarium graminearum mitovirus 1. Phylogenetic analysis indicated that NoMV4 belongs to the genus Unuamitovirus within the family Mitoviridae. The genome of NoOLV1 is 2951 nucleotides in length and encodes an RdRp protein with the highest sequence similarity (64.46% identity) to that of Nigrospora sphaerica ourmia-like virus 1. Phylogenetic analysis suggested that NoOLV1 is a new member of the genus Deltascleroulivirus within the family Botourmiaviridae. This is the first report of the complete genome sequence of a botoulivirus in N. oryzae.