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<p>The fungal genus <i>Fusarium</i> includes several pathogenic species of significant agricultural importance for tobacco cultivation. In this study, we characterized a novel mycovirus, designated "Fusarium commune mononegavirus 1" (FcMV1), isolated from <i>Fusarium commune</i> strain JF-46. The bipartite genome of FcMV1 comprises two negative-sense RNA segments: RNA1 (6,443 nt) and RNA2 (6,301 nt). RNA1 contains a single open reading frame (ORF1, position nt 102–6098) encoding a 224.5-kDa polyprotein with two conserved functional domains: an RNA-dependent RNA polymerase (RdRp) and a mononegavirus mRNA-capping region V. RNA2 has seven putative ORFs (ORF2-ORF8), with ORF6 predicted to encode a nucleocapsid protein, while the remaining ORFs encode hypothetical proteins of unknown function. A multiple sequence alignment revealed 53.5% identity between the FcMV1 polyprotein and its counterpart in Trichoderma harzianum mononegavirus 2, with the nucleocapsid protein showing 33.4% sequence identity. A phylogenetic tree based on RdRp sequences, constructed using the maximum-likelihood method, positioned FcMV1 within a clade with members of the genus <i>Plasmopamonavirus</i> of the family <i>Mymonaviridae</i>. This is the first report of a negative-sense RNA mycovirus infecting <i>F. commune</i>, expanding our understanding of mycovirus diversity in phytopathogenic fungi.</p>

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Identification and characterization of a novel negative-sense RNA virus infecting Fusarium commune, a fungus causing tobacco root rot

  • 期刊论文

  • 发表日期：

    2025年6月21日

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  • Huagang Xiao,
  • Yonghui Peng,
  • Kai Teng,
  • Xiangping Zhou,
  • Yansong Xiao,
  • Zhenyu Deng,
  • Qi Huang,
  • Can Wang,
  • Lu Zheng,
  • Jiatao Xie,
  • Tianbo Liu

摘要

The fungal genus Fusarium includes several pathogenic species of significant agricultural importance for tobacco cultivation. In this study, we characterized a novel mycovirus, designated "Fusarium commune mononegavirus 1" (FcMV1), isolated from Fusarium commune strain JF-46. The bipartite genome of FcMV1 comprises two negative-sense RNA segments: RNA1 (6,443 nt) and RNA2 (6,301 nt). RNA1 contains a single open reading frame (ORF1, position nt 102–6098) encoding a 224.5-kDa polyprotein with two conserved functional domains: an RNA-dependent RNA polymerase (RdRp) and a mononegavirus mRNA-capping region V. RNA2 has seven putative ORFs (ORF2-ORF8), with ORF6 predicted to encode a nucleocapsid protein, while the remaining ORFs encode hypothetical proteins of unknown function. A multiple sequence alignment revealed 53.5% identity between the FcMV1 polyprotein and its counterpart in Trichoderma harzianum mononegavirus 2, with the nucleocapsid protein showing 33.4% sequence identity. A phylogenetic tree based on RdRp sequences, constructed using the maximum-likelihood method, positioned FcMV1 within a clade with members of the genus Plasmopamonavirus of the family Mymonaviridae. This is the first report of a negative-sense RNA mycovirus infecting F. commune, expanding our understanding of mycovirus diversity in phytopathogenic fungi.