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<p>A new negative-stranded ophio-like RNA mycovirus, Rhizoctonia solani mycoophiovirus 1 (RsMOPV1), was isolated from strain JZ56 of <i>Rhizoctonia solani</i> AG-1 IA. The full-length RNA genome of RsMOPV1 is 7,317 nucleotides (nt) in length and consists of a single long open reading frame (ORF). The ORF encodes an RNA-dependent RNA polymerase (RdRp) consisting of 7,138 amino acids, with a predicted molecular mass of 273 kDa. The coding region of the RsMOPV1 genome is flanked by a short structured 3' untranslated region (3'-UTR) of 58 nucleotides and a 5' untranslated region (5'-UTR) of 121 nucleotides. We did not detect any additional segments of RsMOPV1. The nucleotide sequence of the genome of RsMOPV1 shares 74.7% identity with that of Rhizoctonia&#xa0;solani&#xa0;negative-stranded&#xa0;virus 2. RsMOPV1 infections may be associated with hypovirulence and a slow growth rate in <i>R. solani</i>. The RdRp of RsMOPV1 contains five typical RdRp motifs. Phylogenetic analysis demonstrated that RsMOPV1 clusters with members of the previously proposed genus "<i>Mycoophiovirus</i>". </p>

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Genome structure and diversity of a novel ophio-like virus that infects the phytopathogenic fungus Rhizoctonia solani

  • 期刊论文

  • 发表日期：

    2025年7月1日

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  • Yangyi Li,
  • Xingxue Huang,
  • Guolin Zhou,
  • Anhua Ye,
  • Yaohua Deng,
  • Mengting Zhu,
  • Haixiao Wang,
  • Runhua Zhang

摘要

A new negative-stranded ophio-like RNA mycovirus, Rhizoctonia solani mycoophiovirus 1 (RsMOPV1), was isolated from strain JZ56 of Rhizoctonia solani AG-1 IA. The full-length RNA genome of RsMOPV1 is 7,317 nucleotides (nt) in length and consists of a single long open reading frame (ORF). The ORF encodes an RNA-dependent RNA polymerase (RdRp) consisting of 7,138 amino acids, with a predicted molecular mass of 273 kDa. The coding region of the RsMOPV1 genome is flanked by a short structured 3' untranslated region (3'-UTR) of 58 nucleotides and a 5' untranslated region (5'-UTR) of 121 nucleotides. We did not detect any additional segments of RsMOPV1. The nucleotide sequence of the genome of RsMOPV1 shares 74.7% identity with that of Rhizoctonia solani negative-stranded virus 2. RsMOPV1 infections may be associated with hypovirulence and a slow growth rate in R. solani. The RdRp of RsMOPV1 contains five typical RdRp motifs. Phylogenetic analysis demonstrated that RsMOPV1 clusters with members of the previously proposed genus "Mycoophiovirus".