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<p><i>Klebsiella variicola,</i> an emerging pathogen, significantly impacts banana production by causing bacterial sheath rot. Research has shown that phages are effective in treating bacterial infections. In this study, two lytic <i>K. variicola</i> bacteriophages, PZK-KV7 and PZK-KV23, were isolated from a banana plantation area in Guangdong Province, their biological characteristics were examined, and their whole genomes were sequenced. Both phages were found to belong to the genus <i>Sugarlandvirus</i> of the family <i>Demerecviridae</i>. No antibiotic resistance or virulence genes were identified in either of these phages. One-step growth curve analysis revealed that phages PZK-KV7 and PZK-KV23 have a short latency time and a large burst size. pH tolerance tests revealed that both phages remain stable over a broad pH range (pH 4-12). The ability of these phages to inhibit the growth of <i>K. variicola</i> indicates their potential utility for managing <i>K. variicola</i> infections in banana cultivation.</p>

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Characterization and genetic analysis of two sugarlandviruses: Klebsiella variicola lytic phages vB_PZK-KV7 and vB_PZK-KV23

  • 期刊论文

  • 发表日期：

    2025年6月12日

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  • Le Zhong,
  • Yunqian Sun,
  • Yunhao Sun,
  • Guohui Yu,
  • Ping Cheng,
  • Jinshui Zheng,
  • Donghai Peng,
  • Ming Sun

摘要

Klebsiella variicola, an emerging pathogen, significantly impacts banana production by causing bacterial sheath rot. Research has shown that phages are effective in treating bacterial infections. In this study, two lytic K. variicola bacteriophages, PZK-KV7 and PZK-KV23, were isolated from a banana plantation area in Guangdong Province, their biological characteristics were examined, and their whole genomes were sequenced. Both phages were found to belong to the genus Sugarlandvirus of the family Demerecviridae. No antibiotic resistance or virulence genes were identified in either of these phages. One-step growth curve analysis revealed that phages PZK-KV7 and PZK-KV23 have a short latency time and a large burst size. pH tolerance tests revealed that both phages remain stable over a broad pH range (pH 4-12). The ability of these phages to inhibit the growth of K. variicola indicates their potential utility for managing K. variicola infections in banana cultivation.