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<p><i>Stenotrophomonas</i> phage vB_Srh_LBjhp91a was isolated from a karst cave, and its genome was sequenced. This phage has a 63,966-bp circular dsDNA genome (60.7% G + C content) containing 83 predicted open reading frames and two tRNAs. A BLASTn search revealed 80.95% nucleotide sequence identity to <i>Stenotrophomonas</i> phage vB_Sm_QDWS359 (genus <i>Xooduovirus</i>, family <i>Mesyanzhinovviridae</i>). Phylogenetic analysis based on the DNA polymerase and terminase large subunit genes grouped vB_Srh_LBjhp91a with members of the genera <i>Bosavirus</i>, <i>Elanorvirus</i>, and <i>Xooduovirus</i>. Proteomic phylogenetic analysis further clustered it with phage vB_Sm_QDWS359. Sequence comparisons revealed 71.7% average nucleotide identity between phage vB_Srh_LBjhp91a and phage vB_Sm_QDWS359. These findings indicate that phage vB_Srh_LBjhp91a should be considered a member of a new species within the genus <i>Xooduovirus</i>. </p>

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Genomic analysis of the novel Stenotrophomonas phage vB_Srh_LBjhp91a, isolated from a Karst cave in China

  • 期刊论文

  • 发表日期：

    2025年5月29日

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  • Lou Ren,
  • Zheng Fang,
  • Shixia Li,
  • Qingshan Wu,
  • Lan Xiang,
  • Chuangen Lin,
  • Qiuping Liu,
  • Leitao Tan,
  • Qingbei Weng

摘要

Stenotrophomonas phage vB_Srh_LBjhp91a was isolated from a karst cave, and its genome was sequenced. This phage has a 63,966-bp circular dsDNA genome (60.7% G + C content) containing 83 predicted open reading frames and two tRNAs. A BLASTn search revealed 80.95% nucleotide sequence identity to Stenotrophomonas phage vB_Sm_QDWS359 (genus Xooduovirus, family Mesyanzhinovviridae). Phylogenetic analysis based on the DNA polymerase and terminase large subunit genes grouped vB_Srh_LBjhp91a with members of the genera Bosavirus, Elanorvirus, and Xooduovirus. Proteomic phylogenetic analysis further clustered it with phage vB_Sm_QDWS359. Sequence comparisons revealed 71.7% average nucleotide identity between phage vB_Srh_LBjhp91a and phage vB_Sm_QDWS359. These findings indicate that phage vB_Srh_LBjhp91a should be considered a member of a new species within the genus Xooduovirus.