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<p><i>Botryosphaeria dothidea</i> is a prevalent pathogen of woody plants with a global distribution. In this study, we identified a novel mycovirus from the <i>B. dothidea</i> strain ZM200473, which we have tentatively designated “Botryosphaeria dothidea non-segmented dsRNA virus” (BdNSRV1). The genome of BdNSRV1 is composed of dsRNA that spans 2,902 base pairs and contains two non-overlapping open reading frames (ORF1 and ORF2). ORF1 encodes a hypothetical protein consisting of 316 amino acids with a molecular weight of 35.0 kDa, and this protein shares similarity to the coat proteins of several mycoviruses. ORF2 encodes a protein of 561 amino acids with a molecular weight of 66.0 kDa, which includes a conserved RNA-dependent RNA polymerase (RdRp). Sequence comparisons and phylogenetic analysis indicated that BdNSRV1 establishes a well-supported independent clade alongside members of the recently established genus <i>Unirnavirus</i>, being most closely related to Lasiodiplodia pseudotheobromae mycovirus 1 (LpMyV1), with 70.74% amino acid sequence identity in the RdRp. Therefore, BdNSRV1 should be classified as a novel non-segmented dsRNA mycovirus of the genus <i>Unirnavirus</i> and is the first characterized non-segmented virus associated with <i>B. dothidea</i>.</p>

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Molecular characterization of a novel non-segmented double stranded RNA mycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea

  • 期刊论文

  • 发表日期：

    2025年6月6日

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  • Shunpei Xie,
  • Yanan Zhang,
  • Guoqing Xing,
  • Qinzhou Ma,
  • Yashuang Guo,
  • Rui Zang,
  • Chao Xu,
  • Haiyan Wu,
  • Haiqiang Li,
  • Meng Zhang

摘要

Botryosphaeria dothidea is a prevalent pathogen of woody plants with a global distribution. In this study, we identified a novel mycovirus from the B. dothidea strain ZM200473, which we have tentatively designated “Botryosphaeria dothidea non-segmented dsRNA virus” (BdNSRV1). The genome of BdNSRV1 is composed of dsRNA that spans 2,902 base pairs and contains two non-overlapping open reading frames (ORF1 and ORF2). ORF1 encodes a hypothetical protein consisting of 316 amino acids with a molecular weight of 35.0 kDa, and this protein shares similarity to the coat proteins of several mycoviruses. ORF2 encodes a protein of 561 amino acids with a molecular weight of 66.0 kDa, which includes a conserved RNA-dependent RNA polymerase (RdRp). Sequence comparisons and phylogenetic analysis indicated that BdNSRV1 establishes a well-supported independent clade alongside members of the recently established genus Unirnavirus, being most closely related to Lasiodiplodia pseudotheobromae mycovirus 1 (LpMyV1), with 70.74% amino acid sequence identity in the RdRp. Therefore, BdNSRV1 should be classified as a novel non-segmented dsRNA mycovirus of the genus Unirnavirus and is the first characterized non-segmented virus associated with B. dothidea.