Scholarlink

<p>The current study aims to investigate six <i>Quercus</i> species from Northern Pakistan, a biogeographically important region of the Western Himalayas, through DNA barcoding and phylogenetic analysis. Two chloroplast gene markers, <i>mat</i>K and <i>rbc</i>L, were used to amplify and sequence DNA from collected specimens to evaluate interspecific variability and evolutionary relationships. BLAST results and sequence alignments demonstrated the effectiveness of these markers in distinguishing among closely related species. In the <i>mat</i>K<i>,</i> there were 266 conserved, 403 variable, 288 parsimony-informative sites, and 115 singleton sites, while 427 conserved, 210 variable, and 210 parsimony-informative sites were recorded in <i>rbc</i>L region. Phylogenetic trees generated using UPGMA and neighbor-joining methods revealed three strongly supported clades and established genetic affiliations with globally recognized <i>Quercus</i> species. The query <i>Quercus baloot</i> showed a close relationship with reference <i>Q. baloot</i> (LC858530.1<i>)</i>, (OM021739.1)<i>,</i> and (LM652998.1), <i>Quercus dilatata</i> with <i>Q. floribunda</i> (JX860842.1), (MH058671.1), (LC588401.1), and (LC429301.1), <i>Quercus glauca</i> with <i>Q. glauca</i> (JX860839.1), (LC691982.1), (MT129956.1), and (LC644124.1), <i>Quercus incana</i> with <i>Q. incana</i> (OR732696.1), (KY627033.1), (MH551845.1), and (KJ773060.1), <i>Quercus semecarpifolia</i> with <i>Q. semecarpifolia</i> (KX837363.1), (KX837365.1), (MF044963.1), (LM653032.1), and (OM021762.1), and <i>Quercus robur</i> with <i>Q. robur</i> (LT996900.1), (NC046388.1), (PQ999168.1), and (PQ999168.1) with minimum 63 and maximum 100 bootstraps values. In conclusion, this study represents the first comprehensive analysis on genus <i>Quercus</i> from the Northern Pakistan. These findings contribute to accurate species identification and enhance the phylogenetic understanding of <i>Quercus</i> in Pakistan, with implications for the conservation and management of these ecologically and economically important species.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Unraveling oak diversity: molecular characterization and phylogenetic relationships of Quercus species from the western Himalayas

  • 期刊论文

  • 发表日期：

    2026年3月14日

查看全文
  • Ayesha Azeem,
  • Daniel Potter,
  • Abdul Majid,
  • Sajjad Sajjad,
  • Khushi Muhammad,
  • Irfan Ullah,
  • Asif Khan,
  • Abdulwahed Fahad Alrefaei,
  • Sajid Ali

摘要

The current study aims to investigate six Quercus species from Northern Pakistan, a biogeographically important region of the Western Himalayas, through DNA barcoding and phylogenetic analysis. Two chloroplast gene markers, matK and rbcL, were used to amplify and sequence DNA from collected specimens to evaluate interspecific variability and evolutionary relationships. BLAST results and sequence alignments demonstrated the effectiveness of these markers in distinguishing among closely related species. In the matK, there were 266 conserved, 403 variable, 288 parsimony-informative sites, and 115 singleton sites, while 427 conserved, 210 variable, and 210 parsimony-informative sites were recorded in rbcL region. Phylogenetic trees generated using UPGMA and neighbor-joining methods revealed three strongly supported clades and established genetic affiliations with globally recognized Quercus species. The query Quercus baloot showed a close relationship with reference Q. baloot (LC858530.1), (OM021739.1), and (LM652998.1), Quercus dilatata with Q. floribunda (JX860842.1), (MH058671.1), (LC588401.1), and (LC429301.1), Quercus glauca with Q. glauca (JX860839.1), (LC691982.1), (MT129956.1), and (LC644124.1), Quercus incana with Q. incana (OR732696.1), (KY627033.1), (MH551845.1), and (KJ773060.1), Quercus semecarpifolia with Q. semecarpifolia (KX837363.1), (KX837365.1), (MF044963.1), (LM653032.1), and (OM021762.1), and Quercus robur with Q. robur (LT996900.1), (NC046388.1), (PQ999168.1), and (PQ999168.1) with minimum 63 and maximum 100 bootstraps values. In conclusion, this study represents the first comprehensive analysis on genus Quercus from the Northern Pakistan. These findings contribute to accurate species identification and enhance the phylogenetic understanding of Quercus in Pakistan, with implications for the conservation and management of these ecologically and economically important species.