Scholarlink

<p>Glucose is a vital energy source, and its accurate monitoring is crucial for the diagnosis and management of metabolic diseases. Herein, we report a simple colorimetric sensing platform for glucose detection based on MnO<sub>2</sub>/Au-Ag nanozymes, glucose oxidase (GOx), and 3,3′,5,5′-tetramethylbenzidine (TMB). In this cascade system, GOx catalyzes the oxidation of glucose to generate H<sub>2</sub>O<sub>2</sub>, which is subsequently decomposed by MnO<sub>2</sub>/Au-Ag to produce reactive species that oxidize TMB, yielding a visible blue signal. The incorporation of Au-Ag nanoparticles provides additional catalytic sites and promotes interfacial electron transfer, thereby enhancing the catalytic performance of MnO<sub>2</sub>. The platform exhibits a linear response over 0.025-0.20 mM with a detection limit of 0.012 mM. It enables reliable glucose detection in artificial sweat and 80-fold diluted serum samples. In addition, portable nitrocellulose test strips were developed for visual and smartphone-assisted glucose detection. This strategy provides a simple and portable approach for low-concentration glucose analysis and offers potential for point-of-care applications after further validation.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

MnO2/Au-Ag nanozyme-GOx cascade system for sensitive colorimetric glucose detection and test strip applications

  • 期刊论文

  • 发表日期：

    2026年7月4日

查看全文
  • Yuchun Qiao,
  • Yiying Wu,
  • Jiahui He,
  • Wen Chen,
  • Jiaqi Fan,
  • Zhicong Zhang,
  • Jialu Shen,
  • Mengping Su,
  • Qidong Wang,
  • Shuai Li,
  • Yingjie Shen,
  • Jizhen Shang,
  • Hua Wang

摘要

Glucose is a vital energy source, and its accurate monitoring is crucial for the diagnosis and management of metabolic diseases. Herein, we report a simple colorimetric sensing platform for glucose detection based on MnO2/Au-Ag nanozymes, glucose oxidase (GOx), and 3,3′,5,5′-tetramethylbenzidine (TMB). In this cascade system, GOx catalyzes the oxidation of glucose to generate H2O2, which is subsequently decomposed by MnO2/Au-Ag to produce reactive species that oxidize TMB, yielding a visible blue signal. The incorporation of Au-Ag nanoparticles provides additional catalytic sites and promotes interfacial electron transfer, thereby enhancing the catalytic performance of MnO2. The platform exhibits a linear response over 0.025-0.20 mM with a detection limit of 0.012 mM. It enables reliable glucose detection in artificial sweat and 80-fold diluted serum samples. In addition, portable nitrocellulose test strips were developed for visual and smartphone-assisted glucose detection. This strategy provides a simple and portable approach for low-concentration glucose analysis and offers potential for point-of-care applications after further validation.

Graphical Abstract